- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MABN704,Anti-ASS1 Antibody, clone 2C10
产品名称：Anti-ASS1 Antibody, clone 2C10
产品型号：MABN704
Detect Argininosuccinate synthase using this mouse monoclonal antibody, Anti-ASS1 Antibody, clone 2C10 validated for use in western blotting & IHC.
merck millipore,默克密理博,MABN704,Anti-ASS1 Antibody, clone 2C10
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Mk, H, R, M IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABN704 描述 Anti-ASS1 Antibody, clone 2C10 Alternate Names - ASS1
- Argininosuccinate synthase
- Citrulline--aspartate ligase
背景信息 Argininosuccinate synthase (ASS1) or alternatively known by the older name of Citrulline-Aspartate ligase is encoded by the ASS1/ASS gene in humans and is the enzyme that is responsible for the third step in the urea cycle critical to the metabolism of nitrogen. ASS1 catalyzes the synthesis of argininosuccinate from citrulline and aspartate. Argininosuccinate is the immediate precursor of arginine and ASS1 was first identified as the rate limiting step in the liver for this reaction. ASS1 is now recognized to be expressed ubiquitously in mammalian tissues. ASS1 also performs the rate limiting step for NO production in the citrulline-NO cycle as well. Regulation of ASS1 expression can vary depending upon the tissue examined. In the liver, where arginine is hydrolyzed to form urea and ornithine, ASS1 is highly expressed, and various hormones such as glucocorticoids, glucagon, insulin and even high dietary levels of specific amino acids like glutamine regulate expression of ASS1 during development and adult life. In other tissues and in particular in nitric oxide (NO) producing cells, ASS1 is expressed at a low level but induced by proinflammatory cytokines. Interestingly ASS1 expression is also increased in Alzheimer’s disease in both neurons and glia, which also is correlated with iNOS expression and suggests a link between increased NO production and iNOS expression observed in Alzheimer brains. EMD-Millipore’s Anti-ASS1 monoclonal antibody has been tested in western blot on recombinant protein and cell lysates from A431, Raji, MOLT4, Jurkat, A549, NIH3T3, PC-12, and COS7 and in paraffin embedded immunohistochemistry on cervical cancer tissue sections. The antibody has also been tested in direct ELISA on recombinant antigen. 产品信息 格式 Purified 控制 - A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates
演示 Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer. 应用 应用 Detect Argininosuccinate synthase using this mouse monoclonal antibody, Anti-ASS1 Antibody, clone 2C10 validated for use in western blotting & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ASS1 in human cervical cancer and colon cancer tissues.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified recombinant fragment of human ASS1 expressed in E. Coli. 克隆 2C10 宿主 Mouse 同种型 IgG1 品种反应性 MonkeyHumanRatMouse 抗体类型 Monoclonal Antibody 基因符号 - ASS1
- ASS
- CTLN1
纯化方法 Protein G purified UniProt编号 - P00966
产品使用声明 质量保证 Evaluated by Western Blotting in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ASS1 in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,12657,Albumin, Bovine Serum, Fraction V, Crystalline
下一件merck millipore产品：merck millipore,默克密理博,539655,PKCβI, Human, Recombinant, S. frugiperda
相关产品
merck millipore,默克密理博,07-450,Anti-monomethyl-Histone H3 (Lys9) Antibody
merck millipore,默克密理博,ABF208,Anti-NALP12 Antibody
merck millipore,默克密理博,05-1100,Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2
merck millipore,默克密理博,344550,FTI-276 - Calbiochem
merck millipore,默克密理博,12657,Albumin, Bovine Serum, Fraction V, Crystalline
merck millipore,默克密理博,539655,PKCβI, Human, Recombinant, S. frugiperda
merck millipore,默克密理博,05-23-3800,Endothelin 1, Human and Porcine - CAS 117399-94-7 - Calbiochem
merck millipore,默克密理博,AB911,Anti-Oxytocin Antibody
merck millipore,默克密理博,345733,Ganglioside GM₃, Monosialo, Ammonium Salt, Bovine Milk - Calbiochem
merck millipore,默克密理博,553215,RAPIDstain™ Reagent - Calbiochem