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merck millipore,默克密理博,MABN702,Anti-HK2 Antibody, clone 3D3
产品名称：Anti-HK2 Antibody, clone 3D3
产品型号：MABN702
Detect Hexokinase II using this mouse monoclonal antibody, Anti-HK2 Antibody, clone 3D3 validated for use in western blotting, IHC & Flow Cytometry.
merck millipore,默克密理博,MABN702,Anti-HK2 Antibody, clone 3D3
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC, WB, IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MABN702 描述 Anti-HK2 Antibody, clone 3D3 Alternate Names - HK2
- Hexokinase-2
- Hexokinase type II
- HK II
- Muscle form hexokinase
背景信息 There are four hexokinases in most vertebrates, hexokinase I, II, III, and IV; HK2 is hexokinase-II (Hexokinase-2). Hexokinase-2 (HK2) or alternatively Hexokinase type II (HK II) or muscle form hexokinase is the predominant hexokinase enzyme expressed in insulin-responsive tissues like skeletal muscle and other tissues like brain. HK2 is also expressed at high levels in many cancers which is thought to aid in their survival. HK2 is responsible for the phosphorylation of hexoses such as glucose into glucose-6-phosphate; the initial step in carbohydrate metabolism. HK2 is typically associated with mitochondrial membranes. Over expression of HK2 is associated with resistance to cell death and often given the name of the “Warburg Effect” after the initial discover of the relationship between energy metabolism and cell death. While HK2 overexpression has been shown to be one of the mechanisms for the survival of cancers, it has also been shown to facilitate the survival of normal, non-cancerous tissues such as neurons and astrocytes in response to injury and stress. Thus because of its relationship to energy metabolism and its connection with cell death and survival, HK2 is being examined as a potential target for treatment of everything from brain tumors to aging. EMD-Millipore’s Anti-HK2 mouse monoclonal has been tested in western blot on Jurkat, HeLa, and HEK293 cell lysates and in paraffin embedded immunohistochemistry on esophagus and human lung cancer tissues and in flow cytometry on K562 cells in culture. 产品信息 格式 Ascites 控制 - Jurkat, HeLa, and HEK293 cell lysates
演示 Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Detect Hexokinase II using this mouse monoclonal antibody, Anti-HK2 Antibody, clone 3D3 validated for use in western blotting, IHC & Flow Cytometry. 主要应用 - Flow Cytometry
- Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected HK2 in human esophagus cancer and lung cancer tissues.
Flow cytometry analysis: A 1:200-400 dilution from a representative lot detected HK2 in K562 cells.
Optimal working dilutions must be determined by end user.
生物信息 免疫原品种 Purified recombinant fragment of human HK2 expressed in E. Coli. 克隆 3D3 宿主 Mouse 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody 基因符号 - HK2
- HKII
- HXK2
- DKFZp686M1669
纯化方法 Unpurified UniProt编号 - P52789
产品使用声明 质量保证 Evaluated by Western Blotting in Jurkat, HeLa, and HEK293 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected HK2 in Jurkat, HeLa, and HEK293 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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