merck millipore,默克密理博,MABN16,Anti-Beclin-1 (CT) Antibody, clone EPR1733Y, rabbit monoclonal

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merck millipore,默克密理博,MABN16,Anti-Beclin-1 (CT) Antibody, clone EPR1733Y, rabbit monoclonal
产品名称：Anti-Beclin-1 (CT) Antibody, clone EPR1733Y, rabbit monoclonal
产品型号：MABN16
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Beclin-1 (C-term) Antibody, clone EPR1733Y, Rab More>>

merck millipore,默克密理博,MABN16,Anti-Beclin-1 (CT) Antibody, clone EPR1733Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABN16,Anti-Beclin-1 (CT) Antibody, clone EPR1733Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB, ICC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	MABN16
描述	Anti-Beclin-1 (CT) Antibody, clone EPR1733Y, rabbit monoclonal
Alternate Names	beclin 1 (coiled-coil, moesin-like BCL2-interacting protein) beclin 1, autophagy related beclin 1 (coiled-coil, moesin-like BCL2 interacting protein) Coiled-coil myosin-like BCL2-interacting protein ATG6 autophagy related 6 homolog
背景信息	The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).

产品信息
格式	Unpurified
控制	A431 cell lysate
演示	Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Beclin-1 (C-term) Antibody, clone EPR1733Y, Rabbit is an antibody against Beclin-1 (C-term) for use in IH(P), IP, IC & WB.
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunohistochemistry Analysis: A 1:50 dilution from a previous lot detected Beclin-1 in human breast tissue. Immunocytochemistry Analysis: A 1:50 – 1:100 dilution from a previous lot detected Beclin-1 in HeLa cells. Immunoprecipitation Analysis: A 1:50 dilution from a previous lot detected Beclin-1 in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to human Beclin-1 near the C-terminus.
表位	C-terminus
克隆	EPR1733Y
宿主	Rabbit
特异性	This antibody recognizes Beclin-1 near the C-terminus.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Proven to react with human.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_003757.1
基因符号	Protein GT197 ATG6 VPS30 beclin1 beclin-1 GT197
纯化方法	Unpurified
UniProt编号	Q14457
UniProt汇总	FUNCTION: Plays a central role in autophagy By similarity. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. SUBUNIT STRUCTURE: Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with KIAA0831/Barkor. UVRAG and KIAA0831/Barkor form mutually exclusive complexes through direct competition for BECN1-binding. Interacts with BCL2 and BCL2L1. SUBCELLULAR LOCATION: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Note: Interaction with KIAA0831/Barkor promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells By similarity. TISSUE SPECIFICITY: Ubiquitous. SEQUENCE SIMILARITIES: Belongs to the beclin family.

产品使用声明
质量保证	Evaluated by Western Blot in A431 cell lysate. Western Blot Analysis: A 1:5,000 dilution of this antibody detected Beclin-1 in A431 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.