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品种反应性 主要应用 宿主 格式 抗体类型 M, H, R, F ELISA, IHC, WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MABN15 描述 Anti-Rhodopsin Antibody, clone 4D2 Alternate Names - opsin 2, rod pigment
- rhodopsin
- rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant)
- retinitis pigmentosa 4, autosomal dominant
背景信息 Rhodopsin is a multi-pass, G-protein-coupled membrane receptor protein belonging to the G-protein coupled receptor 1 family. Found within rod photoreceptor cells, rhodopsin is activated by light photons in order for the visual transduction cascade to initialize. Rhodopsin is necessary to the forming of images in low light and for the viability of photoreceptor cells after birth. Mutations in rhodopsin expression are the most widely known cause of the human disease retinitis pigmentosa (RP) resulting in gradual loss of vision. At present, there are over 100 known rhodopsin mutations. 产品信息 格式 Purified 控制 - Rat eye tissue
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Rhodopsin Antibody, clone 4D2 is an antibody against Rhodopsin for use in IH, WB, IC, ELISA. 主要应用 - ELISA
- Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Western Blot Analysis: A previous lot of this antibody detected Rhodopsin by WB in goldfish retina lysate, as reported by Knight K, et al.
Immunocytochemistry: A previous lot of this antibody detected Rhodopsin by ICC in goldfish retina tissue, as reported by Knight K, et al
Immunohistochemistry Analysis: 1:500 dilution of this antibody has been shown to detect Rhodopsin in human eye tissue.
ELISA: Specificity of this antibody, from a different lot, was validated in a competitive ELISA, as reported by Laird D, et al.生物信息 免疫原品种 Rat rod outer segments corresponding to rat Rhodopsin at the N-terminus. 表位 N-terminus 克隆 4D2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Rhodopsin at the N-terminus. 同种型 IgG2bκ 品种反应性 MouseHumanRatFish Species Reactivity Note Demonstrated to react with human and rat. Predicted to react with mouse based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_254276
Entrez基因汇总 Visual pigment protein of rod photoreceptors involved in G-protein coupled receptor mediated signaling and phototransduction [RGD]. 基因符号 - Opsin-2
- OPN2
- RHO
- RP4
- CSNBAD
纯化方法 Protein G purfied UniProt编号 - P51489
UniProt汇总 FUNCTION: Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal (By similarity).
SUBUNIT STRUCTURE: Homodimer (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia (By similarity).
TISSUE SPECIFICITY: Rod shaped photoreceptor cells which mediates vision in dim light.
PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore (By similarity).
SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
BIOPHYSICOCHEMICAL PROPERTIES: Absorption: Abs(max)=495 nm产品使用声明 质量保证 Evaluated by Immunohistochemistry in rat eye tissue.
Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Rhodopsin in rat eye tissue.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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