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merck millipore,默克密理博,MABF323,Anti-CD45 (human) Antibody, AlexaFluor488, clone HI30
产品名称：Anti-CD45 (human) Antibody, AlexaFluor488, clone HI30
产品型号：MABF323
Anti-CD45 (human) Antibody, AlexaFluor488, clone HI30 is a highly specific mouse monoclonal antibody, that targets CD45 & has been tested in Flow Cytometry, IHC & western blotting.
merck millipore,默克密理博,MABF323,Anti-CD45 (human) Antibody, AlexaFluor488, clone HI30
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, FC, IHC M AlexaFluor®488 Monoclonal Antibody 描述 产品目录编号 MABF323 Replaces 04-1102 描述 Anti-CD45 (human) Antibody, AlexaFluor®488, clone HI30 Alternate Names - CD45
- Receptor-type tyrosine-protein phosphatase C
- Leukocyte common antigen
- L-CA
- T200
背景信息 CD45, also known as PTPRC, is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells. CD 45 belongs to the protein-tyrosine phosphatase family, receptor class 1/6 subfamily.It also contains 2 fibronectin type-III domains and 2 tyrosine-protein phosphatase domains. CD45 has been shown to play an important role in T-cell activation through the antigen receptor. CD45 also is suggested to act as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one is suggested to affect the substrate specificity of the first domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN. Furthermore, it has been shown to dephosphorylate LYN, and modulate LYN activity. Interestingly, CD45 has been associated with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. CD45 has been shown to bind with GANAB and PRKCSH. Additionally, it interacts with SKAP1 and DPP4, whereas the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Mutations in CD45 have been associated with Multiple Sclerosis (MS) and severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. 产品信息 格式 AlexaFluor®488 演示 Purified mouse conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide and 0.2% (w/v) BSA. 应用 应用 Anti-CD45 (human) Antibody, AlexaFluor®488, clone HI30 is a highly specific mouse monoclonal antibody, that targets CD45 & has been tested in Flow Cytometry, IHC & western blotting. 主要应用 - Western Blotting
- Flow Cytometry
- Immunohistochemistry
应用说明 Western Blotting Analysis: A representative lot detected human CD45 in Western Blotting. (Esser M, et al. 2001.)
Immunohistochemistry Analysis: A representative lot detected human CD45 in Immunohistochemistry. (Friedman T, et al. 1999.)
生物信息 免疫原品种 Immunogen information on this antibody is proprietary information. 克隆 HI30 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 Human 抗体类型 Monoclonal Antibody 基因符号 - CD45
- PTPRC
纯化方法 Affinity UniProt编号 - P08575
产品使用声明 质量保证 The antibody was purified by affinity chromatography, and conjugated with AlexaFluor®488 under optimal conditions. The solution is free of unconjugated AlexaFluor®488 and unconjugated antibody. Alexa Fluor® 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Flow Cytometry Analysis: 5 μL of this antibody detected CD45 in one million human peripheral blood lymphocytes.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
包装信息 数量 100 tests
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