merck millipore,默克密理博,HTS125RTA,Ready-to-Assay™ sst4 Somatostatin Family Receptor Frozen Cells

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merck millipore,默克密理博,HTS125RTA,Ready-to-Assay™ sst4 Somatostatin Family Receptor Frozen Cells
产品名称：Ready-to-Assay™ sst4 Somatostatin Family Receptor Frozen Cells
产品型号：HTS125RTA
Human sst4 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS125RTA,Ready-to-Assay™ sst4 Somatostatin Family Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS125RTA,Ready-to-Assay™ sst4 Somatostatin Family Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	sst4

描述
产品目录编号	HTS125RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ sst₄ Somatostatin Family Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. Somatostatin is a 14 or 28 amino acid regulatory peptide that inhibits hormone secretion from the pituitary, pancreas, and other endocrine sites. A family of 6 GPCRs, sst1, sst2A, sst2B, sst3, sst4 and sst5, mediate the biological activity of somatostatins. The somatostatin receptors couple to Gi to inhibit cAMP production, and also increase MAP kinase signalling. Several tumors have been shown to overexpress somatostatin receptors, and binding of somatostatin to these tumor cells stimulates or inhibits proliferation, depending on the receptor subtypes expressed (Olias et al., 2004). Somatostatin has been implicated in seizure susceptibility in animal models, and activation of sst4 with selective agonists increases seizure activity (Moneta et al., 2002). Millipore’s cloned human sst4-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant sst4 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at sst4.

产品信息
部件	Pack contains 1 vial of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
检测方法	Fluorescent

应用
应用	Human sst4 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_001052
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	sst4
靶子系列	Somatostatin
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.