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merck millipore,默克密理博,MABE61,Anti-macro-H2A1.2 Antibody, clone 14G7
产品名称：Anti-macro-H2A1.2 Antibody, clone 14G7
产品型号：MABE61
Anti-macro-H2A1.2 Antibody, clone 14G7 is a Mouse Monoclonal Antibody for detection of macro-H2A1.2 also known as H2A histone family member Y or Histone macroH2A1 & has been validated in WB, ICC & IHC More>>
merck millipore,默克密理博,MABE61,Anti-macro-H2A1.2 Antibody, clone 14G7
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC, WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MABE61 描述 Anti-macro-H2A1.2 Antibody, clone 14G7 Alternate Names - Core histone macro-H2A.1
- H2A histone family, member Y
- Histone macroH2A1
- Medulloblastoma antigen MU-MB-50.205
- histone macroH2A1.1
- histone macroH2A1.2
背景信息 Histone MacroH2A (mH2A), also known as H2AFY, is a vertebrate-specific histone variant associated with condensed, inactive heterochromatin. This histone variant consists of a histone H2A-like domain fused to a large non-histone region. mH2A has been shown to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. The non-histone region of mH2A is directly involved in the repression of transcription, histone acetylation and blocking SWI/SNF and ACF nucleosome remodeling. This variant histone is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci.
产品信息 格式 Purified 控制 - MCF-7 cell lysate
演示 Purified mouse monoclonal IgG2bλ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-macro-H2A1.2 Antibody, clone 14G7 is a Mouse Monoclonal Antibody for detection of macro-H2A1.2 also known as H2A histone family member Y or Histone macroH2A1 & has been validated in WB, ICC & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Sporn, J.C., et al. (2009). Oncogene. 1–6.)
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Sporn, J.C., et al. (2009). Oncogene. 1–6.)生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human macro-H2A1.2. 克隆 14G7 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001035248
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. 基因符号 - macro-H2A1.2
- H2A.y
- H2A/y
- H2AF12M
- H2AFJ
- MACROH2A1
- MACROH2A1.1
- mH2A1
- macroH2A1.2
纯化方法 Protein G UniProt编号 - O75367
UniProt汇总 FUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 (By similarity).
SUBCELLULAR LOCATION: Nucleus. Chromosome. Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. TISSUE SPECIFICTY: Ubiquitous.
PTM: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.
MISCELLANEOUS: The number of individuals with antibodies against this antigen is 2-fold higher in pediatric patients with cancer compared to healthy controls.
SEQUENCE SIMILARITIES: Contains 1 histone H2A domain.
Contains 1 Macro domain.产品使用声明 质量保证 Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected macro-H2A1.2 on 10 µg of MCF-7 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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