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merck millipore,默克密理博,MABE419,Anti-m3G-cap, m7G-cap Antibody, clone H-20
产品名称：Anti-m3G-cap, m7G-cap Antibody, clone H-20
产品型号：MABE419
Anti-m3G-cap, m7G-cap Antibody, clone H-20 is a mouse monoclonal antibody, validated for use in Dot Blot, IP, western blotting & ICC.
merck millipore,默克密理博,MABE419,Anti-m3G-cap, m7G-cap Antibody, clone H-20
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 A ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABE419 描述 Anti-m3G-cap, m7G-cap Antibody, clone H-20 背景信息 The nucleotide structure named the 2,2,7-trimethylguanosine(m3G)-containing cap structure of small nuclear ribonucleoprotein particles, or U snRNPs is an essential part of mRNA processing. Each snRNP particle consists of one (U1, U2 and U5) or two (U4/U6) snRNA molecules, a common set of eight core proteins (B, B9, D1, D2, D3, E, F and G, also denoted Sm proteins) that are bound to each of the 2,2,7- trimethylguanosine (m3G) cap-containing snRNAs U1, U2, U4 and U5, and several proteins associated specifically with the individual U snRNPs. Except for U6 snRNP, which does not leave the nucleus, the synthesis of these U snRNPs requires the bidirectional transport of the snRNA across the nuclear envelope. The snRNAs U1, U2, U4 and U5 are synthesized in the nucleus with a 7-monomethylguanosine (m7G) cap structure whereas the Sm proteins are stored in the cytoplasm and do not migrate into the nucleus in the absence of bound U snRNA. Instead, newly transcribed U snRNAs are transiently exported into the cytoplasm where the Sm proteins bind the snRNA’s Sm site, to form a ribonucleoprotein complex referred to as the Sm core. Stable association of all Sm proteins is essential for the hypermethylation of the m7G-cap to the m3G-cap structure. After this event and processing of the snRNAs, the mature snRNP particles are transported back to the nucleus in a receptor-and energy-dependent manner and the complete particle is formed. Monoclonal H-20 recognizes both m3G cap containing snRNPs as well as m7G capped structures and it should have a wide application, not only for studying the molecular biology and immunology of the U snRNPs from diverse organisms, but also for the characterization and isolation of m7G-capped transcripts. 产品信息 格式 Purified 演示 Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide. 应用 应用 Anti-m3G-cap, m7G-cap Antibody, clone H-20 is a mouse monoclonal antibody, validated for use in Dot Blot, IP, western blotting & ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunoprecipitation Analysis: A representative lot immunoprecipitated m3G-capped and m7G-capped Xenopus EF-1a, which was spiked into 4 µg of HeLa total RNA.
Western Blotting Analysis: A representative lot from an independent laboratory detected m3G-cap in m3G-capped snRNAs from HeLa nuclear extracts (Bochnig, P., et al. (1987). Eur J Biochem. 168(2):461-467.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated m3G-cap from prefractionated HeLa S100 extracts (Huber, J., et al. (1998). EMBO J. 17(14):4114-4126.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected m3G-cap in m3G-capped U1 snRNPs from digitonin permeabilized HeLa cells (Bochnig, P., et al. (1987). Eur J Biochem. 168(2):461-467.).生物信息 免疫原品种 Human m3G-cap and m7G-cap 克隆 H-20 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 All Species Reactivity Note Predicted to react with all species. 抗体类型 Monoclonal Antibody Entrez基因编号 - NA
纯化方法 Protein G purified UniProt编号 - NA
产品使用声明 质量保证 Evaluated by Dot Blot in in vitro transcribed RNA without and with m7G-cap.
Dot Blot Analysis: 1 µg/mL of this antibody detected m7G-cap in in vitro transcribed RNA containing m7G-cap.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µg
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