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merck millipore,默克密理博,ABS194,Anti-phospho PDHE1-A type I (Ser300) Antibody
产品名称：Anti-phospho PDHE1-A type I (Ser300) Antibody
产品型号：ABS194
This Anti-phospho PDHE1-A type I (Ser300) Antibody is validated for use in WB, IC for the detection of phospho PDHE1-A type I (Ser300).
merck millipore,默克密理博,ABS194,Anti-phospho PDHE1-A type I (Ser300) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Xn, H, M, R, B ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS194 描述 Anti-phospho PDHE1-A type I (Ser300) Antibody Alternate Names - PDHE1-A type I
- Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
背景信息 In many organisms, the pyruvate dehydrogenase complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic, energy-generating pathways, in addition to serving as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition. 产品信息 格式 Affinity Purified 控制 - Dichloroacetate untreated and treated HEK 293 cell lysates
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-phospho PDHE1-A type I (Ser300) Antibody is validated for use in WB, IC for the detection of phospho PDHE1-A type I (Ser300). 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PDHE1-A type I in dichloroacetate untreated and treated HEK 293 cells. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser300. 表位 Phosphorylated Ser300 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes PDHE1-A type I phosphorylated at Ser300. 品种反应性 XenopusHumanMouseRatBovine Species Reactivity Note Demonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Zebra Fish, and Xenopus based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000275
Entrez基因汇总 The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. 基因符号 - PHE1A
- PDHA1
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - P08559
UniProt汇总 FUNCTION: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
CATALYTIC ACTIVITY: Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.
COFACTOR: Thiamine pyrophosphate.
ENZYME REGULATION: E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.
SUBUNIT STRUCTURE: Tetramer of 2 alpha and 2 beta subunits.
SUBCELLULAR LOCATION: Mitochondrion matrix.
TISSUE SPECIFICITY: Ubiquitous.产品使用声明 质量保证 Evaluated by Western Blot in dichloroacetate untreated and treated HEK 293 cell lysates.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PDHE1-A type I on 10 µg of dichloroacetate untreated and treated HEK 293 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 50 µg
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