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merck millipore,默克密理博,MAB5256X,Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated
产品名称：Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated
产品型号：MAB5256X
Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated is an antibody against Neurofilament H for use in IH & IC.
merck millipore,默克密理博,MAB5256X,Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated
- 产品介绍
- merck millipore,默克密理博,MAB5256X,Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Po, R, H ICC, IHC M AlexaFluor®488 Monoclonal Antibody 描述 产品目录编号 MAB5256X 描述 Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor® 488 Conjugated Alternate Names - neurofilament, heavy polypeptide 200kDa
- 200 kDa neurofilament protein
- Neurofilament triplet H protein
- neurofilament, heavy polypeptide
背景信息 Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure; however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. 产品信息 格式 AlexaFluor®488 控制 - Rat Cortex Cells.
演示 Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.1% sodium azide. 应用 应用 Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated is an antibody against Neurofilament H for use in IH & IC. 主要应用 - Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry:
5-10 µg/mL on frozen brain tissue. Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified neurofilament polypeptides (Debus et al., 1983). 表位 Unknown 克隆 NE14 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Neurofilament H. 同种型 IgG1 品种反应性 PigRatHuman Species Reactivity Note Human, Porcine and Rat. Reactivity with other species has not been tested. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_066554.2
Entrez基因汇总 Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq].
基因符号 - NFH
- NEFH
- NF-H
纯化方法 Protein G purfied UniProt编号 - P12036
UniProt汇总 FUNCTION:Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
POLYMORPHISM: The number of repeats is shown to vary between 29 and 30.
DISEASE:Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. Ref.8
SIMILARITY: Belongs to the intermediate filament family.
产品使用声明 质量保证 Immunocytochemical Analysis: 1:250 dilution of this lot detected Neurofilament-H in rat cortex cell culture. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8ºC from date of receipt. 包装信息 数量 40 µg
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