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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M IHC, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB9566 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Otx2 Antibody 产品信息 格式 Serum 演示 Rabbit serum. Liquid. 应用 应用 Anti-Otx2 Antibody detects level of Otx2 & has been published & validated for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western blot: 1:500-1:4,000. The antibody reacts with a doublet at 36 kDa in adult mouse retina extract.
Immunohistochemistry: 1:500-1:4,000. Suggested fixative is 4% PFA-perfused or immersion fixed tissue. Suggested permeablization method is Triton X100 and blocking buffer is 5% serum in PBS. Suggested incubation time is overnight at 2-8°C.
Immunocytochemistry
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Full length recombinant human OTX2 (excluding the first 5 amino acids). 宿主 Rabbit 特异性 The antibody recognizes OTX2. The antibody will also react with OTX1 due to amino acid sequence homology between the two proteins. OTX2 is expressed earlier than OTX1 and therefore the antibody can be used to identify OTX2 during development. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_172337.1
- NM_021728.2
Entrez基因汇总 This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants may exist, but their full length sequences have not been determined. 基因符号 - MGC45000
- OTX2
- MCOPS5
UniProt编号 - P32243
UniProt汇总 FUNCTION: SwissProt: P32243 # Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
SIZE: 289 amino acids; 31636 Da
SUBCELLULAR LOCATION: Nucleus (Probable).
TISSUE SPECIFICITY: Expressed in brain.
DEVELOPMENTAL STAGE: Embryo.
DISEASE: SwissProt: P32243 # Defects in OTX2 are the cause of syndromic microphthalmia 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities such as cardiac defects, facial clefts, microcephaly and hydrocephaly. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures.
SIMILARITY: SwissProt: P32243 ## Belongs to the paired homeobox family. Bicoid subfamily. & Contains 1 homeobox DNA-binding domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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