merck millipore,默克密理博,FCMAB101A4,Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, FC	M	AlexaFluor®488	Monoclonal Antibody

描述
产品目录编号	FCMAB101A4
商名	Milli-Mark
描述	Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated
Alternate Names	BrdU
背景信息	Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody's special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.

产品信息
格式	AlexaFluor®488
控制	After incorporation of BrdU, all DNA containing species
演示	Presentation needs to say: Purified mouse monoclonal IgG2a supernatant conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.

应用
应用	Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.
主要应用	Immunocytochemistry Flow Cytometry

生物信息
免疫原品种	Bromodeoxyuridine
克隆	Bu-1
宿主	Mouse
特异性	Specifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.
同种型	IgG2a
品种反应性	Human
Species Reactivity Note	Tested on Human. Predicted to react with all species.
抗体类型	Monoclonal Antibody
基因符号	BrdU
纯化方法	Protein G purfied

产品使用声明
质量保证	Evaluated by flow cytometry with Jurkat Cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 6 months at 2-8°C from date of receipt. Protect from light.

包装信息
数量	100 tests

merck millipore,默克密理博,FCMAB101A4,Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated

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