merck millipore,默克密理愽
  • merck millipore,默克密理博,MABC666,Anti-EIF4B Antibody, clone 1F5

  • merck millipore,默克密理博,MABC666,Anti-EIF4B Antibody, clone 1F5

    产品名称：Anti-EIF4B Antibody, clone 1F5
    产品型号：MABC666
    Detect eIF4B using this mouse monoclonal antibody, Anti-EIF4B Antibody, clone 1F5 validated for use in western blotting, ICC, Flow Cytometry & IHC.

    merck millipore,默克密理博,MABC666,Anti-EIF4B Antibody, clone 1F5

  • 产品介绍
  • merck millipore,默克密理博,MABC666,Anti-EIF4B Antibody, clone 1F5

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, HIHC, WB, ICC, FCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MABC666
    描述 Anti-EIF4B Antibody, clone 1F5
    Alternate Names
    • eIF-4B
    • Eukaryotic translation initiation factor 4B
    背景信息 The eukaryotic translation initiation factor 4B (eIF4B, eIF-4b) is one of several proteins involved in the initiation of eukaryotic translation. Together with other proteins EIF4B forms a complex with the 40S ribosomal subunit and Met-tRNA that ultimately allows for translation to begin. EIF4B contains two RNA binding domains, one binds RNA and the other specifically binds the 18S portion of the small (40S) ribosomal subunit RNA. EIF4B act as an anchor and then interacts with eIF4A, a helicase to help unwind and position the RNA for translation. EIF4B must be phosphorylated on Ser422 in order for translation to occur; the phosphorylation is mediated by S6K1/S6K2 kinases and or p90S6 kinase (RSK) in a growth factor dependent manner. Activation of S6K or RSK proteins requires PDK1 phosphorylation as well, thus two great signaling pathways, the mTOR/Pi3K and MAPK pathways converge on EIF4B to control its phosphorylation and subsequent activity. EMD-Millipore’s Anti-EIF4B mouse monoclonal antibody has been tested in western blot against recombinant human EIF4B protein as well lysates from a variety of human cell lines. In addition, the antibody has been successfully tested in flow cytometry on HeLa over expressing EIF4B and in fluorescent immunocytochemistry on mouse NIH3T3 cells in culture.
    产品信息
    格式 Purified
    控制
    • A549, A431, HEK293, HeLa, Jurkat, K562, and MCF-7 cell lysates
    演示 Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.
    应用
    应用 Detect eIF4B using this mouse monoclonal antibody, Anti-EIF4B Antibody, clone 1F5 validated for use in western blotting, ICC, Flow Cytometry & IHC.
    主要应用
    • Immunohistochemistry
    • Western Blotting
    • Immunocytochemistry
    • Flow Cytometry
    应用说明 Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected EIF4B in colon cancer tissue.

    Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected EIF4B in NIH/3T3 cells.

    Flow Cytometry Analysis: A 1:50 dilution from a representative lot detected EIF4B in non serum starved HeLa cells.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Purified recombinant fragment of human EIF4B expressed in E. Coli.
    克隆 1F5
    宿主 Mouse
    同种型 IgG2b
    品种反应性 MouseHuman
    抗体类型 Monoclonal Antibody
    基因符号
    • EIF-4B
    纯化方法 Protein G purified
    UniProt编号
    • P23588
    产品使用声明
    质量保证 Evaluated by Western Blot in A549, A431, HEK293, HeLa, Jurkat, K562, and MCF-7 cell lysates.

    Western Blot Analysis: A 1:500-2000 dilution of this antibody detected EIF4B in A549, A431, HEK293, HeLa, Jurkat, K562, and MCF-7 cell lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MABC666,Anti-EIF4B Antibody, clone 1F5

上一件merck millipore产品：merck millipore,默克密理博,07-067,Anti-Calcineurin/PP2B Aα Antibody
下一件merck millipore产品：merck millipore,默克密理博,ECM505,QCM Chemotaxis Cell Migration Assay, 24-well (3 µm), fluorimetric


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