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merck millipore,默克密理博,491002,Notch Transcription Factor Inhibitor, SAHM1 - Calbiochem
产品名称：Notch Transcription Factor Inhibitor, SAHM1 - Calbiochem
产品型号：491002
The Notch Transcription Factor Inhibitor, SAHM1 controls the biological activity of NOTCH1 trans-activation complex. This small molecule/inhibitor is primarily used for Inflammation/Immunology applica More>>
merck millipore,默克密理博,491002,Notch Transcription Factor Inhibitor, SAHM1 - Calbiochem
- 产品介绍
重要规格表
经验配方 C₉₄H₁₆₂N₃₆O₂₃S• 7CF₃CO₂H•13H₂O 描述 概述 A cell-permeable, stabilized hydrocarbon-stapled α-helical peptide that targets a critical protein-protein interface and prevents assembly in the NOTCH1 trans-activation complex. Soluble FITC-SAHM1 was found to bind the pre-formed RAMANK-CSL complex (Kd = 0.12 µM) competitively with MAML1, whereas the unmodified FITC-MAML1 (21-36) peptide bound the complex with markedly diminished affinity in an in vitro pull down assay. Treatment of KOPT-K1 cell lines with 20 µM of compound results in the suppression of NOTCH1 target genes, HES4, DTX1, and HES1. A consistent repressive effect on NOTCH1-activated gene expression by this compound was observed across a panel of human T-ALL cell lines, containing diverse mutant NOTCH1 alleles. In HeLa cells, this peptide also inhibited the expression of ICN1 (IC50 = 6.56 µM) dose-dependently in a luciferase reporter-gene assay, as well as that of β-lactamase (IC50 < 2.5 µM) in a second reporter-gene assay. Furthermore, treatment with this peptide inhibits NOTCH-mediated proliferation in cultured cells at 15 µM, and in a mouse model of T-ALL cells at 30 mg/kg twice daily. 产品目录编号 491002 品牌系列 Calbiochem®
同义词 AcβA1a-ERLRRRI*LCR*HHST (*=S5) 参考 参考 Moellering, R.E., et al. 2009. Nature 462, 182. 产品信息 形式 Off-white solid Hill配方 C₉₄H₁₆₂N₃₆O₂₃S• 7CF₃CO₂H•13H₂O 化学配方 C₉₄H₁₆₂N₃₆O₂₃S• 7CF₃CO₂H•13H₂O Molar Mass 3228.9 肽序列 Acetyl-βAla-ERLRRRI*LCR*HHST
* = Two S5 molecules (a non-natural alkenyl amino acid) stapled together with a double bond生物信息 纯度 ≥93% by HPLC 理化信息 溶解度 DMSO 肽序列 Acetyl-βAla-ERLRRRI*LCR*HHST
* = Two S5 molecules (a non-natural alkenyl amino acid) stapled together with a double bond储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Following reconstitution, aliquot and freze (20°C). Stock solutions are stable for up to 3 months at -20°C.
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