merck millipore,默克密理博,MAB6021,Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
R, M	WB, ELISA, ICC	M	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MAB6021
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5
背景信息	Aldosterone synthase (or 18-hydroxylase) is a steroid hydroxylase cytochrome P450 oxidase enzyme involved in the generation of aldosterone. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. (Pascoe, L, et al. (1992) "Mutations in the human CYP11B2 (aldosterone synthase) gene causing corticosterone methyloxidase II deficiency." PNAS, USA. 89:496-5000).

产品信息
格式	Culture Supernatant
演示	Unpurified tissue culture supernatant containing 50% glycerol and 0.1% sodium azide as a preservative.

应用
应用	This Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5 is validated for use in ELISA, IC, IH(P), WB for the detection of Cytochrome P450 Aldosterone Synthase.
主要应用	Western Blotting ELISA Immunocytochemistry
应用说明	Western blot: recognizes ~48kDa band in adrenal zona glomerulosa mitochondrial preparations. The enzyme is expressed best in adrenal zona glomerulosa tissues, expression in kidney is virtually undetectable. Immunohistochemistry: 1:50-1:200 , ABC, enzymatic detection; clone works best with STF fixed tissues that are paraffin embedded. STF is available from Streck Laboratories, Formalin fixation has been successfully reported but working dilutions are much lower (see Fujisawa, et al). Recommended counter stain is Gill's Haematoxylin. Immunocytochemistry: acetone fixed cells, 1:20-1:200. ELISA Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Aldo-1-MAP (aa 175-192) (KVRQNARGSLTMDVQQSL-MAP)
克隆	1G5
宿主	Mouse
特异性	Rat cytochrome p450 aldosterone synthase
同种型	IgG1κ
品种反应性	RatMouse
抗体类型	Monoclonal Antibody
UniProt编号	P30099

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

包装信息
数量	500 µL

merck millipore,默克密理博,MAB6021,Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5

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