merck millipore,默克密理博,MAB5386,Anti-Nerve Growth Factor Receptor (p75) Antibody, clone ME20.4

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merck millipore,默克密理博,MAB5386,Anti-Nerve Growth Factor Receptor (p75) Antibody, clone ME20.4
产品名称：Anti-Nerve Growth Factor Receptor (p75) Antibody, clone ME20.4
产品型号：MAB5386
Anti-Nerve Growth Factor Receptor (p75) Antibody, clone ME20.4 detects level of Nerve Growth Factor Receptor (p75) & has been published & validated for use in FC, IC, IH, IH(P), IP, RIA & WB.

merck millipore,默克密理博,MAB5386,Anti-Nerve Growth Factor Receptor (p75) Antibody, clone ME20.4

产品介绍

merck millipore,默克密理博,MAB5386,Anti-Nerve Growth Factor Receptor (p75) Antibody, clone ME20.4

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Rc, Ca, H, Po, Rb, Sh, Fe, Pm	WB, FC, ICC, IHC	M	Purified	Monoclonal Antibody

描述
产品目录编号	MAB5386
Replaces	04-1111
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Nerve Growth Factor Receptor (p75) Antibody, clone ME20.4
Alternate Names	p75 NGFR
背景信息	The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells

产品信息
格式	Purified
控制	Brain tissue
演示	Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.

应用
应用	Anti-Nerve Growth Factor Receptor (p75) Antibody, clone ME20.4 detects level of Nerve Growth Factor Receptor (p75) & has been published & validated for use in FC, IC, IH, IH(P), IP, RIA & WB.
主要应用	Western Blotting Flow Cytometry Immunocytochemistry Immunohistochemistry
应用说明	Western blot: RIPA lysates of human HS294T cells; 1 μg/mL; recognizes ~69-75kDa band depending upon gel conditions. Immunohistochemistry: 5-10 μg/mL on frozen tissue. Immunohistochemistry on formalin-fixed, paraffin embedded sections. Immunocytochemistry: 5-10 μg/mL EM Immunohistochemistry {Caneva, et al 1995}. Immunoabsorptive purification of NGF-receptors. Inhibition of NGF binding to p-75NGFR Immunoprecipitation of p75 FACS: unfixed cells RIA Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Human melanoma cell line (Ross, 1984).
表位	within a.a. 1-160, extracellular domain of h p75.
克隆	ME20.4
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Reacts with the low affinity nerve growth factor receptor (NGFR p75). The epitope recognized by the antibody maps within the third or fourth cysteine-rich repeats of the NGFR molecule. Reactivity has not been observed with rat, chicken, or mouse NGFR.
同种型	IgG1
品种反应性	RaccoonCanineHumanPigRabbitSheepFelinePrimate
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_002507.2
Entrez基因汇总	Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
基因符号	CD271 NGFR p75(NTR) TNFRSF16 Gp80-LNGFR
非反应性品种	Rat Chicken Mouse
纯化方法	Protein A purfied
UniProt编号	P08138
UniProt汇总	FUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SIZE: 427 amino acids; 45183 Da SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.