merck millipore,默克密理博,05-825R,Anti-PDGFR-β Antibody, clone 4A56, rabbit monoclonal

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merck millipore,默克密理博,05-825R,Anti-PDGFR-β Antibody, clone 4A56, rabbit monoclonal
产品名称：Anti-PDGFR-β Antibody, clone 4A56, rabbit monoclonal
产品型号：05-825R
Anti-PDGFR-β Antibody, clone 4A56 is an antibody against PDGFR-β for use in WB & IP.

merck millipore,默克密理博,05-825R,Anti-PDGFR-β Antibody, clone 4A56, rabbit monoclonal

产品介绍

merck millipore,默克密理博,05-825R,Anti-PDGFR-β Antibody, clone 4A56, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H, M	WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	05-825R
Replaces	04-397
描述	Anti-PDGFR-β Antibody, clone 4A56, rabbit monoclonal
Alternate Names	platelet-derived growth factor, B chain PDGF subunit B PDGF, B chain Platelet-derived growth factor B chain Platelet-derived growth factor beta polypeptide Platelet-derived growth factor, beta polypeptide (oncogene SIS) platelet-derived growth factor 2 platelet-derived growth factor beta platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
背景信息	PDGFR (Platelet-Derived Growth Factor Receptor) is a receptor tyrosine kinase for the PDGF family of growth factors. PDGF homo and hetero- dimmers (dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB)) bind to the two structurally related a and b PDGF Receptors (PDGFRa and PDGFRb). The type A PDGF Receptor binds all three PDGFs with high affinity, whereas the type B PDGF Receptor is selective for PDGF-BB. This binding results in the dimerization of the receptor and the subsequent kinase activation of the receptor tyrosine kinase (RTK) and its autophosphorylation. These phosphorylations allow for various proteins, such as PI3 Kinase, PLCg, and Grb2, to bind through their SH2 domains and transduce signals into the cell. The activation of PDGFFR triggers the intracellular loop and activates Ras, Erk1/2, and ROS. PDGFb has been implicated in embryonic development, angiogenesis, and development of tumors of glial origin.

产品信息
格式	Purified
控制	NIH/3T3 cell lysate
演示	Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Anti-PDGFR-β Antibody, clone 4A56 is an antibody against PDGFR-β for use in WB & IP.
主要应用	Western Blotting
应用说明	Immunoprecipitation Analysis: 0.4 µg from a previous lot immunoprecipitated PDGFR-β from BALB/3T3/A31 cells.

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human PDGFR-β at the protein kinase domain.
表位	Protein kinase domain
克隆	clone 4A56
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes PDGFR-β at the protein kinase domain.
同种型	IgG
品种反应性	HumanMouse
Species Reactivity Note	Demonstrated to react with mouse. Predicted to react with human based on 100% sequence homology.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002600
Entrez基因汇总	This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq].
基因符号	PDGFR-β Becaplermin PDGF-2 PDGF2 SIS SSV becaplermin c-sis PDGFRβ
纯化方法	Protein A
UniProt编号	P09619
UniProt汇总	FUNCTION: Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE: Homodimer, and heterodimer with PDGFRA. Interacts with APS. The autophosphorylated form interacts directly with SHB and with PIK3C2B, maybe indirectly. Interacts with GRB10. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. INVOLVEMENT IN DISEASE: Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML). Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia. Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1. Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein. Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.

产品使用声明
质量保证	Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: 1:4,000 dilution of this antibody detected PDGFR-β on 10 µg of NIH/3T3 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.