merck millipore,默克密理博,MAB5324,Anti-Polysialic Acid-NCAM Antibody, clone 2-2B

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merck millipore,默克密理博,MAB5324,Anti-Polysialic Acid-NCAM Antibody, clone 2-2B
产品名称：Anti-Polysialic Acid-NCAM Antibody, clone 2-2B
产品型号：MAB5324
Detect Polysialic Acid-NCAM using this Anti-Polysialic Acid-NCAM Antibody, clone 2-2B validated for use in IC, IH, RIA & WB.

merck millipore,默克密理博,MAB5324,Anti-Polysialic Acid-NCAM Antibody, clone 2-2B

产品介绍

merck millipore,默克密理博,MAB5324,Anti-Polysialic Acid-NCAM Antibody, clone 2-2B

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Vrt, H, R, M	WB, ICC, IHC	M	Ascites	Monoclonal Antibody

描述
产品目录编号	MAB5324
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Polysialic Acid-NCAM Antibody, clone 2-2B
Alternate Names	PSA-NCAM
背景信息	Polysialic acid (PSA) is a long, linear homopolymer that is attached to the neural cell adhesion molecule (NCAM). It has the remarkable ability to down-regulate a wide variety of contact-dependent cell interactions. This activity is believed to stem from the ability of the charged polymer to occupy a large hydrated volume and thereby cause a direct physical hindrance of cell–cell contact. The regulated expression of PSA has been shown to promote a reduction in cell interactions that can create permissive conditions for changes in the structure of a variety of tissues. The broadest expression of PSA occurs on precursor cells during early development and plays a role in promotion of the migration of these cells after cell division. In most cases, PSA expression is lost after the migration process is completed. However, the axons of neurons often retain PSA to bundle,sprout, and branch appropriately during axon path-finding.

产品信息
格式	Ascites
控制	Positive control tissue: Young Rat Hippocampus, human hippocampus.
演示	Mouse monoclonal IgM in buffer containing 50% glycerol, no preservatives.

应用
应用	Detect Polysialic Acid-NCAM using this Anti-Polysialic Acid-NCAM Antibody, clone 2-2B validated for use in IC, IH, RIA & WB.
主要应用	Western Blotting Immunocytochemistry Immunohistochemistry
应用说明	Western Blotting: A 1:500-1:1,000 concentration of a previous lot was used in WB. Immunocyto/histochemistry: A 1:200-1:400 dilution of a previous lot of this antibody worked on live and fixed cells, tissue sections either frozen or fixed with any kind of fixative. (Rougon et al., 1986; Theodosis et al., 1991, 1999). It is not recommend that the antibody be used on paraffin embedded tissue since it gives variable results. Radioimmunoassay (RIA) : A previous lot of this antibody was used in RIA. (Figarella-Branger et al., 1996) Cell sorting and cell panning (Ben-Hur et al., 1998) Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Viable Meningococcus group B (strain 355).
克隆	2-2B
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Reacts with alpha 2-8 linked neuraminic acid (NeuAc-alpha 2-8) n with n >10. This polymer is usually termed polysialic acid (PSA). In vertebrates PSA is linked to neural cell adhesion molecule (NCAM, CD56), in bacteria it is associated with capsula of meningococcus strain group B.
同种型	IgM
品种反应性	VertebratesHumanRatMouse
Species Reactivity Note	The monoclonal works well on all species expressing PSA-NCAM including mouse.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_000615.5
基因符号	MSK39 NCAM1 CD56 NCAM-140 NCAM NCAM-120
纯化方法	Unpurified
UniProt编号	P13591
UniProt汇总	FUNCTION: SwissProt: P13592 # This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.\| P13591 # This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. SIZE: 761 amino acids; 83770 Da SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. DOMAIN: SwissProt: P13592 SIMILARITY: SwissProt: P13592 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. \| P13591 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

产品使用声明
质量保证	Routinely evaluated by Immunohistochemistry on hippocampus. Immunohistochemistry(paraffin) Analysis: PSA-NCAM (cat. # MAB5324) representative staining pattern/morphology on human hippocampus. Tissue pretreated with EDTA pH 8, antigen retrieval. Antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as plasma membrane staining in neurons. Optimal Staining With Epitope Retrieval: Alzheimer’s Hippocampus
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Routinely evaluated by Immunohistochemistry on hippocampus.

Immunohistochemistry(paraffin) Analysis:
PSA-NCAM (cat. # MAB5324) representative staining pattern/morphology on human hippocampus. Tissue pretreated with EDTA pH 8, antigen retrieval. Antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as plasma membrane staining in neurons.
Optimal Staining With Epitope Retrieval: Alzheimer’s Hippocampus

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.