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品种反应性 主要应用 宿主 格式 抗体类型 M, H, R ELISA, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB4305 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Oct-4 Antibody, clone 9E3.2 概述 This antibody is suitable for immunoblotting and ELISA. It is NOT recommended for immunofluorescence. Alternate Names - Oct-3
- Octamer-4
背景信息 Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state. 产品信息 格式 Purified 控制 - Pluripotent human embryonic stem (ES) cells, and mouse brain lysate.
演示 Purified mouse monoclonal IgG1 liquid in buffer containing 0.02M PBS, 0.25 NaCl pH 7.6 with 0.1% Sodium Azide. 应用 应用 Anti-Oct-4 Antibody, clone 9E3.2 is an antibody against Oct-4 for use in WB, ELISA. 主要应用 - ELISA
- Western Blotting
应用说明 (Note: For ICC analysis, please order Catalog No. MAB4419).
ELISA:
A prevoius lot of this antibody was used in ELISA.
Western blot:
Recommended dilution of 10 µg/mL is suggested (MW 44 kDa)
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Recombinant protein corresponding to amino acids 143-359 of Human Oct-4 克隆 9E3.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody detects both Oct-4A and Oct4B isoforms.
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known. Oct4 expression has been seen in mouse somatic stem cells, including those in the brain (Lengner, C.J, et al., 2007).同种型 IgG1 品种反应性 MouseHumanRat Species Reactivity Note Mouse. Expected to react with human and rat based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002701.4
基因符号 - Oct-4
- POU5F1
- Oct-3
- MGC22487
- Oct4
- OTF4
- OTF3
- OCT3
- OCT4
纯化方法 Protein A purfied UniProt编号 - Q01860
UniProt汇总 FUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene.
SIZE: 360 amino acids; 38571 Da
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.产品使用声明 质量保证 Evaluated by Western Blot in Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of a previous lot detected Oct-4 on 10 μg of Mouse Brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µg
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