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merck millipore,默克密理博,MAB424R,Anti-PCNA Antibody, clone PC10
产品名称：Anti-PCNA Antibody, clone PC10
产品型号：MAB424R
Anti-PCNA Antibody, clone PC10 is a mouse monoclonal antibody for detection of PCNA also known as Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor & has been validated in F More>>
merck millipore,默克密理博,MAB424R,Anti-PCNA Antibody, clone PC10
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Vrt, Inv WB, FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB424R 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-PCNA Antibody, clone PC10 Alternate Names - DNA Polymerase delta Processivity Factor
- Proliferating Cell Nuclear Antigen
背景信息 Proliferating cell nuclear antigen (PCNA) is a 36 kDa molecule that is highly conserved between species. PCNA was first identified as the antigen for a subpopulation of autoantibodies in patients with systemic lupus erythematosus (Miyachi, 1978; Takasaki, 1984; Ogata, 1987). It has since been determined that PCNA serves as a co-factor for DNA polymerase delta in S-phase as well as during DNA synthesis associated with mechanisms involved in DNA damage repair (Tan, 1987; Bravo, 1987). The temporal specificity of PCNA expression makes it an ideal marker for cell proliferation. PCNA begins to accumulate during the G1 phase of the cell cycle, is most abundant during the S phase, and declines during the G2/M phase (Kurki, 1988). Since the half-life of PCNA exceeds 20 hours, it may be possible to detect the protein in non-cycling cells. 产品信息 格式 Purified 控制 - Tonsil or reactive lymph node tissue
演示 Purified immunoglobulin (ion-exchange chromatography). Liquid. 应用 应用 Anti-PCNA Antibody, clone PC10 is a mouse monoclonal antibody for detection of PCNA also known as Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor & has been validated in FC, IP, WB,IHC(P). 主要应用 - Western Blotting
- Flow Cytometry
应用说明 Western blot: 1:250-1:1000. Nuclear extracts are preferred over whole cell protein extracts.
Immunohistochemistry: 1:20-1:200. Recommended for paraffin embedded tissue sections only. May be used on material fixed in a wide range of fixatives including formalin (buffered and unbuffered), methacarn and Bouin's reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air-drying overnight on poly-L-lysine coated slides is recommended. 60 minute incubation at 25°C with standard ABC technique is recommended.
Immunoprecipitation
Indirect Flow Cytometry
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Rat PCNA made in the protein A vector pR1T2T 克隆 PC10 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Clone PC10 recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells (Kurki, 1988), proliferating cells in solid tumors (Smetana, 1983), and blast cells in leukemia patients (Takasaki, 1984). Immunohistochemistry with clone PC10 has been used to study the expression of PCNA in paraffin sections of normal tissues and lymphoid neoplasms (Hall, 1990). In proliferating cells, PCNA staining pattern is predominantly nuclear. By western blot, the antibody detects a polypeptide migrating at 36 kDa with an isoelectric point of 4.8. 同种型 IgG2a 品种反应性 VertebratesInvertebrates 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_182649.1
- NM_002592.2
Entrez基因汇总 The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. 基因符号 - MGC8367
- PCNA
- Cyclin
- cyclin
UniProt编号 - P12004
UniProt汇总 FUNCTION: SwissProt: P12004 # This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
SIZE: 261 amino acids; 28769 Da
SUBUNIT: Homotrimer. Interacts with KCTD10 (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH.
SUBCELLULAR LOCATION: Nucleus.
PTM: Upon methyl methanesulfonate-induced DNA damage, mono- ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligase SHPRH, whih is required for DNA repair.
DISEASE:SwissProt: P12004 # Antibodies are present in sera from patients with systemic lupus erythematosus.
SIMILARITY: SwissProt: P12004 ## Belongs to the PCNA family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2 to 8°C for up to 6 months after date of receipt. 包装信息 数量 100 µg
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