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merck millipore,默克密理博,MAB4211,Anti-CD34 Class I Antibody, clone B1-3C5
产品名称：Anti-CD34 Class I Antibody, clone B1-3C5
产品型号：MAB4211
Detect CD34 Class I using this Anti-CD34 Class I Antibody, clone B1-3C5 validated for use in FC & IF.
merck millipore,默克密理博,MAB4211,Anti-CD34 Class I Antibody, clone B1-3C5
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB4211 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-CD34 Class I Antibody, clone B1-3C5 产品信息 格式 Purified 演示 The antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry. 应用 应用 Detect CD34 Class I using this Anti-CD34 Class I Antibody, clone B1-3C5 validated for use in FC & IF. 主要应用 - Flow Cytometry
应用说明 Identification of CD34 positive cells is important for the diagnosis and classification of acute myeloid leukemia (AML). It is especially useful in patients with poorly differentiated myeloblasts, as well as in myeloblastic crises of chronic granulocytic leukemia. It has been recommended that this antibody be used as part of a panel for screening of leukemia (Batinic et al., 1989).
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:25
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl of buffer.
2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems.生物信息 免疫原品种 KG1 human acute myelogenous cell line. 克隆 B1-3C5 宿主 Mouse 特异性 This antibody belongs to CD34 (assigned by the Third International Workshop on Leukocyte Differentiation Antigens, Oxford, 1987; Hogg & Horton, 1987). It reacts with a 120kD glycoprotein found on progenitor cell populations in human bone marrow and foetal liver (Katz et al., 1985). This antigen is normally restricted to immature human haemopoietic precursor cells (Peschel & Koller, 1989).
Cell reactivity (Tindle et al., 1985):
Normal: No reaction with normal peripheral lymphocytes, granulocytes, monocytes, erythrocytes and platelets. Reacts with <4% of cells in normal bone marrow.
Clinical: Reaction
Acute myeloid leukemia (AML, M1 and M2) positive
Null lymphoblastic leukemia (Null ALL) positive
Common acute lymphoblastic leukemia (CALL) positive
Myeloblastic and lymphoblastic crises of chronic
granulocytic leukemia (CGL) positive
Chronic myeloid leukemia (CML) negative
T cell acute lymphoblastic leukemia (TALL) negative
B cell acute lymphoblastic leukemia (BALL) negative
Chronic lymphocytic leukemia (CLL) negative
Cell lines:
Reacts with early myeloblast line KG1. No reaction with null, B or T lymphoid, erythroid or later myeloid cell lines.同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001773.2
- NM_001025109.1
Entrez基因汇总 CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM] 基因符号 - CD34
UniProt编号 - P28906
UniProt汇总 FUNCTION: SwissProt: P28906 # Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
SIZE: 385 amino acids; 40716 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells.
PTM: Highly glycosylated. & Phosphorylated on serine residues by PKC.
DISEASE:SwissProt: P28906 # Abnormal CD34 expression in leukemogenesis.
SIMILARITY: SwissProt: P28906 ## Belongs to the CD34 family.产品使用声明 销售限制 This product is not available for sale in the United States. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES. Product stable for 6 months when stored as directed.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.包装信息 数量 100 µg
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