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merck millipore,默克密理博,MAB5272,Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324
产品名称：Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324
产品型号：MAB5272
This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
merck millipore,默克密理博,MAB5272,Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, M WB, ICC, IHC R Purified Monoclonal Antibody 描述 产品目录编号 MAB5272 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 Alternate Names - N-CAM L1
- CD171
背景信息 Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts 产品信息 格式 Purified 控制 - Brain tissue
演示 Rat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide. 应用 应用 This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blotting:
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Rats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice. 克隆 324 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 特异性 Monoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells. 同种型 IgG 品种反应性 RatMouse Species Reactivity Note Mouse and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_032504.3
Entrez基因汇总 This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. 基因符号 - L1CAM
- VCAM1
- INCAM-100
- MGC99561
- CD106
- DKFZp779G2333
纯化方法 Protein A purfied UniProt编号 - P11627
UniProt汇总 FUNCTION: SwissProt: P19320 # Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
SIZE: 739 amino acids; 81276 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
DOMAIN: SwissProt: P19320 Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
PTM: Sialoglycoprotein.
DISEASE: SwissProt: P19320 # May play an important role in the genesis of atherosclerosis and rheumatoid arthritis.
SIMILARITY: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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