merck millipore,默克密理博,AB5472-50UL,Anti-Glycine Receptor α3 Subunit Antibody

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
R, M	IHC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB5472-50UL
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Glycine Receptor α3 Subunit Antibody
Alternate Names	GLRa3

产品信息
格式	Affinity Purified
控制	CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示	Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

应用
应用	This Anti-Glycine Receptor α3 Subunit Antibody is validated for use in IH for the detection of Glycine Receptor α3 Subunit.
主要应用	Immunohistochemistry
应用说明	Western blot: 1:200 on rat brain membranes. Immunohistochemistry on rat brain floating sections and mouse brain floating sections (trypsin pretreatment is recommended). Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	A purified peptide corresponding to amino acid residues 355-368 of rat GlyRa3 (Accession P24524).
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes Glycine receptor alpha3 (GlyRalpha3) protein. The epitope is not similar to any other known proteins.
品种反应性	RatMouse
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_006529.2 NM_001042543.1
基因符号	GLRA3
UniProt编号	O75311
UniProt汇总	FUNCTION: SwissProt: O75311 # The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing). SIZE: 464 amino acids; 53800 Da SUBUNIT: Pentamer composed of alpha and beta subunits (By similarity). SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely distributed throughout the central nervous system. DOMAIN: SwissProt: O75311 The N-terminal domain carries structural determinants essential for agonist and antagonist binding. TM2 is thought to form the innert wall of the chloride channel. The cytoplasmic loop is an important determinant of channel inactivation kinetics. SIMILARITY: Belongs to the ligand-gated ionic channel (TC 1.A.9) family. MISCELLANEOUS: The alpha subunit binds strychnine.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

包装信息
数量	50 µL

merck millipore,默克密理博,AB5472-50UL,Anti-Glycine Receptor α3 Subunit Antibody

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