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merck millipore,默克密理博,MAB3790,Anti-β-Catenin Antibody, clone 9F12.2
产品名称：Anti-β-Catenin Antibody, clone 9F12.2
产品型号：MAB3790
Detect β-Catenin also known as beta catenin with Anti-β-Catenin Antibody, clone 9F12.2 (Mouse Monoclonal Antibody), that has been demonstrated to work in WB.
merck millipore,默克密理博,MAB3790,Anti-β-Catenin Antibody, clone 9F12.2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3790 Replaces 04-1002 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-β-Catenin Antibody, clone 9F12.2 背景信息 Beta-catenin, the vertebrate homologue of Drosophila segment polarity gene armadillo, and key element of the Wnt signal transduction pathways, is important in embryogenesis, cell transformation, and intercellular adhesion including the adhesion, motility and metastasis of cancer cells. Thus, catenin protein expression, regulation and localization are potentially important markers to predict motility and invasiveness of epithelial neoplasms. Besides adhesion functions, beta-catenin binds to the tumor suppressor gene APC. APC mutation disturbs the equilibrium and cytoplasmic levels of free beta-catenin level in the cell and may have a role in tumorigenesis. 产品信息 格式 Purified 控制 - Western blot positive control: A431 cell line
演示 Protein A purified immunoglobulin. Liquid in 0.02M Phosphate Buffer, 0.25M NaCl, with 0.1% sodium azide. 应用 应用 Detect β-Catenin also known as beta catenin with Anti-β-Catenin Antibody, clone 9F12.2 (Mouse Monoclonal Antibody), that has been demonstrated to work in WB. 主要应用 - Western Blotting
应用说明 Western blot: 2-5 μg/mL
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide corresponding to residues 27-37 of Human beta catenin. Sequence is 100% identical in mouse, rat, Xenopus, zebrafish, chicken, porcine, and gerbil. 克隆 9F12.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes human beta catenin. This antibody detects a band of approximately 97 kDa in Western blot analysis of A431 cell lysate. 同种型 IgG1κ 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001904.3
- NM_001098209.1
- NM_001098210.1
Entrez基因汇总 Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM] 基因符号 - DKFZp686D02253
- CTNNB1
- FLJ25606
- CTNNB
- Beta-catenin
- PRO2286
- FLJ37923
UniProt编号 - P35222
UniProt汇总 FUNCTION: SwissProt: P35222 # Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
SIZE: 781 amino acids; 85497 Da
SUBUNIT: Two separate pools are found in the cytoplasm: one is PSEN1/cadherin/catenin complex which anchors to the actin cytoskeleton. The other pool is part of a large complex containing AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. Wnt- dependent activation of DVL antagonizes the action of GSK3B. When GSK3B activity is inhibited the complex dissociates, CTNNB1 is dephosphorylated and is no longer targeted for destruction. The stabilized protein translocates to the nucleus, where it binds TCF/LEF-1 family members, TBP, BCL9 and possibly also RUVBL1 and DUPLIN. Binds CTNNBIP and EP300. CTNNB1 forms a ternary complex with LEF1 and EP300 that is disrupted by CTNNBIP1 binding (By similarity). Interacts with the PDZ domain of TAX1BP3; this interaction inhibits the transcriptional activity of CTNNB1 (By similarity). Interacts with AJAP1, BAIAP1, CARM1, CTNNA3, CXADR and PCDH11Y. Binds SLC9A3R1. Interacts with GLIS2. Interacts with SLC30A9 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 promotes nuclear translocation.
TISSUE SPECIFICITY: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheats. Expressed in colon.
PTM: Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr- 41 to Ser-37 and Ser-33. & EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. & Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X (Probable). Its ubiquitination leads to its subsequent proteasomal degradation.
DISEASE: "SwissProt: P35222 # Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life. & Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor. & Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. & A chromosomal rearrangement involving CTNNB1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1."
SIMILARITY: SwissProt: P35222 ## Belongs to the beta-catenin family. & Contains 12 ARM repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 12 months from date of receipt. 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB1886,Anti-Nidogen Antibody, G1 domain, clone JF6
下一件merck millipore产品：merck millipore,默克密理博,217691,CDC25 Phosphatase Inhibitor I, BN82002 - CAS 396073-89-5 - Calbiochem
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