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Replacement Information Replacement Information ABN99 is the recommended replacement for catalog number 06-641 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN99 Replaces 06-641 描述 Anti-phospho NR1 (Ser897) Antibody Alternate Names - glutamate [NMDA] receptor subunit zeta-1
- glutamate receptor, ionotropic, N-methyl D-aspartate 1
- N-methyl-D-aspartate receptor subunit NR1
- glutamate [NMDA] receptor subunit zeta 1
- N-methyl-D-aspartate receptor channel, subunit zeta-1
背景信息 NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory. 产品信息 格式 Affinity Purified 控制 - Rat brain microsomal tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-phospho NR1 (Ser897) Antibody is validated for use in WB, IP, IH for the detection of phospho NR1 (Ser897). 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: 1:50 dilution from a previous lot detected NR1 in normal rat hippocampus.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated NR1 from rat brain microsomal RIPA lysate.
Western Blot Analysis: 2 µg/mL from a previous lot detected NR1 in Lambda phosphatase untreated and treated rat brain microsomal tissue lysate.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to rat NR1 when phosphorylated at Ser897. 表位 Phosphorylated Ser897 宿主 Rabbit 特异性 This antibody recognizes NR1 when phosphorylated at Ser897. 品种反应性 MouseHumanRat Species Reactivity Note Demonstrated to react with rat. Predicted to react with mouse and human based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - Q05586
基因符号 - NMDA1
- NMDAR1
- NMD-R1
- NR1
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - Q05586
UniProt汇总 FUNCTION: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors By similarity.
SUBUNIT STRUCTURE: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ By similarity. Interacts with LRFN1 and LRFN2 By similarity.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein By similarity. Cell junction › synapse › postsynaptic cell membrane By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density By similarity. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities By similarity.
PTM NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity By similarity.
SEQUENCE SIMILARITIES: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. [View classification]产品使用声明 质量保证 Evaluated by Western Blot in rat brain microsomal tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected NR1 on 10 µg of rat brain microsomal tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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