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merck millipore,默克密理博,MAB3576,Anti-Caldesmon Antibody, smooth muscle, clone N5/22
产品名称：Anti-Caldesmon Antibody, smooth muscle, clone N5/22
产品型号：MAB3576
Detect Caldesmon using this Anti-Caldesmon Antibody, smooth muscle, clone N5/22 validated for use in IP, WB, IC, IH(P).
merck millipore,默克密理博,MAB3576,Anti-Caldesmon Antibody, smooth muscle, clone N5/22
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rb, B, H, Po ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3576 Replaces 04-590 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Caldesmon Antibody, smooth muscle, clone N5/22 背景信息 Caldesmon is a protein component of the thin filaments of smooth muscle myofibrils. It
is also localized in the stress fibers of fibroblasts. Caldesmon was identified as a
Ca2+/Calmodulin-binding protein with molecular weight of 120-150kDa (H-Caldesmon)
and 70-80kDa (L-Caldesmon). H-Caldesmon is the primary isoform in smooth muscle
while L-Caldesmon is most abundant in non-muscle cells. Caldesmon is capable of
binding two calmodulin molecules, one at either end of the protein. Additionally,
Caldesmon is an actin, myosin, and tropomyosin-binding protein. Human L-Caldesmon
is a protein of 538 amino acids with mobility of 80kDa. In vitro, L-Caldesmon inhibits
the actomyosin ATPase in an F-Actin-dependent manner. L-Caldesmon may play an
important function in motile processes such as secretion and organelle movement.产品信息 格式 Purified 控制 - POSITIVE CONTROL:
Smooth muscle (e.g. aterial tunica media). Negative control: any nonmuscle tissue (e.g. arterial tunica adentitial).
演示 Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide. 应用 应用 Detect Caldesmon using this Anti-Caldesmon Antibody, smooth muscle, clone N5/22 validated for use in IP, WB, IC, IH(P). 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western blot. Suggested blocking buffer is TBS-Tween with 2% BSA. Suggested dilution buffer is TBS-Tween with 0.05% sodium azide. Preferred gel percentage is 7% and/or 4-20% (or similar) gradient gel.
Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made) follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum. The antibody has also been used successfully on methyl-Carnoy fixed tissue.
Immunocytochemistry
Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Crude smooth muscle extract from normal human adult uterus. 表位 smooth muscle 克隆 N5/22 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Caldesmon, smooth muscle. By Western blot the antibody recognizes a protein of 150-kDa. 同种型 IgG1 品种反应性 RabbitBovineHumanPig 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_033157.2
- NM_004342.5
- NM_033138.2
- NM_033139.2
- NM_033140.2
Entrez基因汇总 This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. 基因符号 - H-CAD
- CALD1
- Caldesmon
- CAD
- L-CAD
- CDM
- MGC21352
- NAG22
UniProt编号 - Q05682
UniProt汇总 FUNCTION: SwissProt: Q05682 # Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
SIZE: 793 amino acids; 93250 Da
SUBCELLULAR LOCATION: Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) (By similarity).
TISSUE SPECIFICITY: High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
DOMAIN: SwissProt: Q05682 The N-terminal part seems to be a myosin/calmodulin- binding domain, and the C-terminal a tropomyosin/actin/calmodulin- binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
PTM: In non-muscle cells, phosphorylation by CDC2 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization (By similarity).
SIMILARITY: Belongs to the caldesmon family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt 包装信息 数量 100 µg
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