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merck millipore,默克密理博,MAB3568,Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone ID8
产品名称：Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone ID8
产品型号：MAB3568
This Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone ID8 is validated for use in IP, WB, IC, IH(P) for the detection of Myosin.
merck millipore,默克密理博,MAB3568,Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone ID8
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rb, B, H, Po ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3568 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone ID8 产品信息 格式 Purified 控制 - POSITIVE CONTROL: Smooth muscle (e.g. aterial tunica media). Negative control: any nonmuscle tissue (e.g. arterial tunica adentitial).
演示 Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide. 应用 应用 This Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone ID8 is validated for use in IP, WB, IC, IH(P) for the detection of Myosin. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western blot (see application notes below). Suggested blocking buffer is TBS-Tween with 2% BSA. Suggested dilution buffer is TBS-Tween with 0.05% sodium azide. Preferred gel percentage is 5% (see application notes).
Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made) follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum.
Immunocytochemistry
Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG.
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR MAB3568
WESTERN BLOT
To achieve good resolution of myosin heavy chain isoforms with distinct molecular weight (200 - 2004 kDa), the following procedure should be followed: 1). Pyrophoshate extraction buffer for sample preparation (see below); run SDS-PAGE in 5% gel. Important: for better resolution of the MHC bands, use electrophoretic buffer with pH 8.2 (i.e. 0.1 less than standard), and prepare resolving gel (5%) with pH 9.0 (not 8.8 as usual). Also help thorough degasing of the resolving gel mixture (H2O, acrylamide, EDTA, pH 9.0, before (!) adding SDS, TEMED and APS). Run SDS-PAGE longer than after the dye front runs off (use 200 kDa MW markers and let it's 200 kDa band run at least to the middle of 8X8 gel (using a big size gel (not the mini-gel!) will enhance the quality of MHC band resolution).
Pyrophosphate extraction buffer: (40mM Na4P2O7x10H2O, 1mM MgCl2, 1mM EGTA (add KOH to dissolve EGTA), PMSF, pH 9.5). To extract acto-myosin from tissues/cells, shake minced tissue or cells in cold extraction buffer 1 hr on ice bath (0oC), centrifuge @10,000g for 10 min at +2-8 oC, take supernatant and mix it 1:1 with standard Laemmli sample buffer, boil, run SDS-PAGE in 5% gel (see above).生物信息 免疫原品种 Crude smooth muscle extract from normal human adult uterus. 表位 smooth muscle heavy chain, SM1 & SM2 克隆 ID8 (also referred to as SMMS-1) 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Smooth muscle myosin, heavy chain. By Western blot the antibody recognizes a protein of 200-204 kDa. 同种型 IgG1 品种反应性 RabbitBovineHumanPig 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_152994.2
基因符号 - FLJ16566
- CCDC128
- MGC111781
UniProt编号 - Q6ZMI0
UniProt汇总 SIZE: 780 amino acids; 88314 Da 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8*C in undiluted aliquots up to 6 months after date of receipt 包装信息 数量 100 µg
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