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merck millipore,默克密理博,MAB3448,Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9
产品名称：Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9
产品型号：MAB3448
Use Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 (Mouse Monoclonal Antibody) validated in ELISA, ICC, IHC, IP, WB to detect Heterochromatin Protein-1 β also known as HP1b.
merck millipore,默克密理博,MAB3448,Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, ELISA, ICC, IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MAB3448 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 Alternate Names - HP1b
背景信息 Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.产品信息 格式 Ascites 控制 - HeLa, 293 or NIH/3T3 cell lysates
演示 Liquid. Contains no preservative. 应用 应用 Use Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 (Mouse Monoclonal Antibody) validated in ELISA, ICC, IHC, IP, WB to detect Heterochromatin Protein-1 β also known as HP1b. 主要应用 - Western Blotting
- ELISA
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blot: 1:500-1:5,000
Immunofluorescence: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
Immunoprecipitation: 1:500 - 1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Recombinant mouse HP1beta. 克隆 1MOD-1A9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with Heterochromatin protein-1 b (HP1b). 同种型 IgG1 品种反应性 MouseHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_007622.3
Entrez基因汇总 Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing.[supplied by OMIM] 基因符号 - HP1B
- CBX1
- HP1-BETA
纯化方法 Unpurified UniProt编号 - P83917
UniProt汇总 FUNCTION: SwissProt: P45973 # Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Can interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
SIZE: 191 amino acids; 22225 Da
SUBUNIT: Interacts with SUV420H1 and SUV420H2 (By similarity). Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with MIS12 and C20orf127.
SUBCELLULAR LOCATION: Nucleus. Centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
PTM: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper- phosphorylated during mitosis.
SIMILARITY: SwissProt: P45973 ## Contains 2 chromo domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µL
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