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merck millipore,默克密理博,05-1502,Anti-CENP-E Antibody, clone177
产品名称：Anti-CENP-E Antibody, clone177
产品型号：05-1502
Use Anti-CENP-E Antibody, clone177 (Mouse Monoclonal Antibody) validated in ICC, IHC, WB, IP to detect CENP-E also known as centromere protein E (312kD).
merck millipore,默克密理博,05-1502,Anti-CENP-E Antibody, clone177
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 05-1502 描述 Anti-CENP-E Antibody, clone177 Alternate Names - centromere protein E
- centromere protein E, 312kDa
- kinesin family member 10
- centromere protein E (312kD)
- Kinesin-related protein CENPE
- Centromere autoantigen E (312kD)
- Centromeric protein E
背景信息 Centromeric protein E (CENP-E) is a mitosis-specific kinesin belonging to the kinesin-7 family. CENP-E, along with cytoplasmic dyenin, is one of two main microtubule motors known to localize at kinetochores. During mitosis, CENP-E has an active role in the migration of chromosomes toward the metaphase plate, and is directly responsible for microtubule capture and stabilization by kinetochores. Reduction in CENP-E expression results in the high occurrence of whole chromosome missegregation, and its removal or inhibition leads to the presence of unattached chromosomes near the spindle pole as well as a significant reduction in the number of microtubules captured by kinetochores. 产品信息 格式 Purified 控制 - HeLa cells
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-CENP-E Antibody, clone177 (Mouse Monoclonal Antibody) validated in ICC, IHC, WB, IP to detect CENP-E also known as centromere protein E (312kD). 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry Analysis:
1:100 dilution from a representative lot of this antibody detected CENP-E in kidney and malignant melanoma tissue.
Western Blot Analysis: A representative lot of this antibody was used by an independent laboratory in WB. (Yen, T.J., et al. (1991). EMBO J. 10(5):1245–1254.)
生物信息 免疫原品种 Native protein corresponding to human CENP-E. 表位 Unknown 克隆 177 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes CENP-E. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001804
Entrez基因汇总 Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq]. 基因符号 - CENP-E
- CENP-E3
纯化方法 Protein G purfied UniProt编号 - Q02224
UniProt汇总 FUNCTION: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule-dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle.
SUBUNIT STRUCTURE: Monomer. Interacts with CENPF, SEPT7, KIF18A, BUB1B kinase and PRC1.
SUBCELLULAR LOCATION: Kinetochore. Cytoplasm › cytoskeleton › spindle. Centromere. Note: Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.
DOMAIN: The protein is composed of a N-terminal kinesin-motor domain involved in the chromosome movements, a long coil-coiled region involved in the homodimerization and an inhibitory C-tail involved in autoinhibition of the N-terminal catalytic part (By similarity).
PTM: The C-teminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor (By similarity).
Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore.
SEQUENCE SIMILARITIES: Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
Contains 25 LRR (leucine-rich) repeats.产品使用声明 质量保证 Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: 1:500 dilution of this antibody detected CENP-E in HeLa cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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