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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB M Purified Monoclonal Antibody 描述 产品目录编号 04-002 品牌系列 Upstate
商名 - Upstate
描述 Anti-SOCS1 Antibody, clone 4H1 Alternate Names - suppressor of cytokine signaling 1, CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP3
- suppressor of cytokine signaling 1
- CIS1
- CISH1
- JAB
- SOCS-1
- SSI-1
- SSI1
产品信息 格式 Purified 应用 应用 Anti-SOCS1 Antibody, clone 4H1 is an antibody against SOCS1 for use in IP & WB. 主要应用 - Western Blotting
应用说明 Immunoblot Analysis: 1:1,000 dilution of this antibody detected SOCS1 in KG-1 cell lysate.
Immunoprecipitation (IP): 10 μg of this antibody immunoprecipitated SOCS1 from 200 μL of cell extract in RIPA buffer.生物信息 免疫原品种 mouse SOCS1 recombinant GST fusion protein encompassing amino acids 2-78. 克隆 4H1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes SOCS1 同种型 IgG1 品种反应性 MouseHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_003745.1
Entrez基因汇总 This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. 基因符号 - JAB
- SOCS1
- SSI-1
- Cish1
- TIP-3
- TIP3
- CISH1
- SSI1
- CIS1
- SOCS-1
纯化方法 Protein G purfied UniProt编号 - O35716
UniProt汇总 FUNCTION: SwissProt: O15524 # SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate-recognition component of a SCF- like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
SIZE: 211 amino acids; 23551 Da
SUBUNIT: Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV (By similarity). Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(VHL) E3 ubiquitin ligase complex which contains Elongin BC complex, CUL5, RBX1 and SOCS1 (By similarity). Interacts with TRIM8 (By similarity). Interacts with CUL2.
TISSUE SPECIFICITY: Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
DOMAIN: SwissProt: O15524 The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition. & The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box (By similarity).
SIMILARITY: Contains 1 SH2 domain. & Contains 1 SOCS box domain.产品使用声明 质量保证 Routinely evaluated by immunoblot. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at 2° to 8°C from date of shipment. 包装信息 数量 100 µg
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