merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB3234,Anti-Cytokeratin 18 Antibody, clone RGE53

  • merck millipore,默克密理博,MAB3234,Anti-Cytokeratin 18 Antibody, clone RGE53

    产品名称：Anti-Cytokeratin 18 Antibody, clone RGE53
    产品型号：MAB3234
    Anti-Cytokeratin 18 Antibody, clone RGE53 is an antibody against Cytokeratin 18 for use in FC, IC, IH & WB.

    merck millipore,默克密理博,MAB3234,Anti-Cytokeratin 18 Antibody, clone RGE53

  • 产品介绍
  • merck millipore,默克密理博,MAB3234,Anti-Cytokeratin 18 Antibody, clone RGE53

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Rb, Ca, Ch, H, Ht, M, Po, RWB, FC, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB3234
    Replaces 04-586
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Cytokeratin 18 Antibody, clone RGE53
    背景信息 Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
    产品信息
    格式 Purified
    控制
    • Skin , Pancreas/tumor
    演示 Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
    应用
    应用 Anti-Cytokeratin 18 Antibody, clone RGE53 is an antibody against Cytokeratin 18 for use in FC, IC, IH & WB.
    主要应用
    • Western Blotting
    • Flow Cytometry
    • Immunocytochemistry
    • Immunohistochemistry
    不建议的应用
    • Immunohistochemistry (Paraffin)
    应用说明 Western blot

    Immunohistochemistry on fresh frozen or acetone fixed tissue sections. Formalin fixed, paraffin embedded tissues are not recommended for RGE53.

    Immunocytochemistry: (10μg/mL) Light fixation with 4% formalin, 2.5% PFA ( 5-10 minutes room temperature) or acetone. {See, Barrett, et al 2001} Permeabilization with 0.2% triton X-100 in blocking buffer if using formalin or PFA fixations.

    Flow cytometry: Acetone fixed/ethanol fixed cells {See Mezosi, E et al, 2002}.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Cytoskeletal preparation from HeLa cells
    克隆 RGE53
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Specifically recognizes glandular epithelial cells from gastro-intestinal tract, respiratory tract and urogenetical tract. As well as endocrine and exocrine tissues and mesothelial cells. It is unreactive with stratified squamous epithelia (esophagus, epidermis, ectocervix). In transitional epithelium of the bladder RGE53 only reacts with the superficial (umbrella) cells. RGE53 can distinguish adenocarcinomas and mesotheliomas from squamous cell carcinoma and non-epithelial tumours. The antibody is directed against 45 kD cytokeratin protein (cytokeratin 18). Cross reactivity with; Keratin from most mammals, birds.
    同种型 IgG1
    品种反应性 RabbitCanineChickenHumanHamsterMousePigRat
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_199187.1
    • NM_000224.2
    Entrez基因汇总 KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
    基因符号
    • CK-18
    • KRT18
    • K18
    • Keratin-18
    • CYK18
    • Cytokeratin-18
    纯化方法 Protein A purfied
    UniProt编号
    • P05783
    UniProt汇总 FUNCTION: SwissProt: P05783 # Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
    SIZE: 430 amino acids; 48058 Da
    SUBUNIT: Heterotetramer of two type I and two type II keratins. Keratin-18 associates with keratin-8. Interacts with the thrombin- antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD.
    SUBCELLULAR LOCATION: Cytoplasm, perinuclear region.
    TISSUE SPECIFICITY: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
    PTM: Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. & Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.DISEASE:SwissProt: P05783 # Defects in KRT18 are a cause of cryptogenic cirrhosis [MIM:215600].
    SIMILARITY: SwissProt: P05783 ## Belongs to the intermediate filament family.
    MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB3234,Anti-Cytokeratin 18 Antibody, clone RGE53

上一件merck millipore产品：merck millipore,默克密理博,539132,Protease Inhibitor Cocktail Set II - Calbiochem
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