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merck millipore,默克密理博,MAB3408,Anti-Tubulin Antibody, beta, clone KMX-1
产品名称:Anti-Tubulin Antibody, beta, clone KMX-1
产品型号:MAB3408
This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.
merck millipore,默克密理博,MAB3408,Anti-Tubulin Antibody, beta, clone KMX-1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, A, H, M WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB3408 Replaces 04-1049 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Tubulin Antibody, beta, clone KMX-1 Alternate Names - tubulin, beta, 4
- Tubulin beta-4
- Tubulin beta-III
- tubulin, beta 3
- beta 3
- beta
背景信息 Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues. 产品信息 格式 Purified 控制 - MCF7 Cell lysate
- HeLa Cell lysate
- A431 Cell lysate
演示 Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide. 应用 应用 This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry:
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.
Western blot:
0.05-0.5 μg/mL
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Tubulin purified from polycephalum myxamoebae 克隆 KMX-1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only. 同种型 IgG2b 品种反应性 RatAllHumanMouse Species Reactivity Note Myxomycetes, yeast, fungi, plants, insects, nematodes, parasites and mammalians. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_006086.2
基因符号 - beta-4
- TUBB3
- TUBB4
- MC1R
纯化方法 Protein A purfied UniProt编号 - Q13509
UniProt汇总 FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
SIZE: 450 amino acids; 50433 Da
SUBUNIT: Dimer of alpha and beta chains.
DOMAIN: SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
SIMILARITY: Belongs to the tubulin family.产品使用声明 质量保证 Routinely evaluated by Western Blot on A431 lysates.
Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt. 包装信息 数量 50 µg
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