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merck millipore,默克密理博,MAB339,Anti-GABA A Receptor α1 chain Antibody, NT, clone BD24
产品名称：Anti-GABA A Receptor α1 chain Antibody, NT, clone BD24
产品型号：MAB339
Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 is an antibody against GABA A Receptor α1 chain for use in IH, IP & WB.
merck millipore,默克密理博,MAB339,Anti-GABA A Receptor α1 chain Antibody, NT, clone BD24
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Po, Av, B, H WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB339 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-GABA A Receptor α1 chain Antibody, NT, clone BD24 产品信息 格式 Purified 演示 Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. 应用 应用 Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 is an antibody against GABA A Receptor α1 chain for use in IH, IP & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry: 10-20 μg/mL * See protocol below.
Western blot: 20 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB339
IMMUNOHISTOCHEMISTRY
1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C.
2) Sections should be incubated floating in suitable small vials.
3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature.
4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v).
5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C
6) Wash sections in Buffer A.
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in Buffer A.
9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips.生物信息 免疫原品种 Purified GABA/benzodiazepine receptor from bovine cortex. 表位 N-terminus 克隆 BD24 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with the alpha 1-chain of the GABAA receptor in bovine and human but not rat 同种型 IgG1 品种反应性 PigAvianBovineHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000806.4
Entrez基因汇总 GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. 基因符号 - EJM
- GABRA1
- ECA4
- GABA
非反应性品种 - Feline
- Rat
UniProt编号 - P14867
UniProt汇总 FUNCTION: SwissProt: P14867 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
SIZE: 456 amino acids; 51802 Da
SUBUNIT: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P14867 # Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME) [MIM:606904]. JME is a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks. & Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. ECA4 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood.
SIMILARITY: SwissProt: P14867 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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