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品种反应性 主要应用 宿主 格式 抗体类型 R, H, Ht, Po WB, ELISA M Ascites Monoclonal Antibody 描述 产品目录编号 MAB331 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-SNAP-25 Antibody, clone SP14 产品信息 格式 Ascites 演示 Liquid. Contains no preservative. 应用 应用 Detect SNAP-25 using this Anti-SNAP-25 Antibody, clone SP14 validated for use in ELISA, WB, IH(P). 主要应用 - Western Blotting
- ELISA
应用说明 Immunohistochemistry at 1:500-1:5,000
Formalin fixed (< 4 weeks), paraffin embedded
* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.
Frozen
Western blotting at 1:100-1:1,000
ELISA at 1:500
It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Crude synaptic immunoprecipitate (Human) 克隆 SP14 宿主 Mouse 特异性 SNAP-25 (26-27 kDa). MAB331 is reactive with fusion protein from COS cells, but not with fusion protein from bacterial systems. 同种型 IgG1 品种反应性 RatHumanHamsterPig 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_130811.1
- NM_003081.2
Entrez基因汇总 Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. 基因符号 - bA416N4.2
- SNAP25
- SNAP
- dJ1068F16.2
- SNAP-25
- FLJ23079
- RIC-4
- SUP
- SEC9
- RIC4
UniProt编号 - P60880
UniProt汇总 FUNCTION: SwissProt: P60880 # t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.
SIZE: 206 amino acids; 23315 Da
SUBUNIT: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9, RIMS1, SNAP25BP and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1 (By similarity).
TISSUE SPECIFICITY: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
PTM: Palmitoylated (By similarity).
SIMILARITY: SwissProt: P60880 ## Belongs to the SNAP-25 family. & Contains 2 t-SNARE coiled-coil homology domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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