merck millipore,默克密理博,06-896,Anti-GFP (Green Fluorescent Protein) Antibody

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merck millipore,默克密理博,06-896,Anti-GFP (Green Fluorescent Protein) Antibody
产品名称：Anti-GFP (Green Fluorescent Protein) Antibody
产品型号：06-896
Anti-GFP (Green Fluorescent Protein) Antibody detects level of GFP (Green Fluorescent Protein) & has been published & validated for use in IC & WB.

merck millipore,默克密理博,06-896,Anti-GFP (Green Fluorescent Protein) Antibody

产品介绍

merck millipore,默克密理博,06-896,Anti-GFP (Green Fluorescent Protein) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Vrt	WB, ICC	Ch	Purified	Polyclonal Antibody

描述
产品目录编号	06-896
品牌系列	Upstate
商名	Upstate
描述	Anti-GFP (Green Fluorescent Protein) Antibody
Alternate Names	Green fluorescent protein
背景信息	Green fluorescent protein (GFP) is a 29 kDa fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488 nm), GFP strongly fluoresces (Emission max: 507 nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells.

产品信息
格式	Purified
控制	Rat brain lysate, cells expressing GFP protein.
演示	Purified chicken polyclonal IgY from egg yolk in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

应用
应用	Anti-GFP (Green Fluorescent Protein) Antibody detects level of GFP (Green Fluorescent Protein) & has been published & validated for use in IC & WB.
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry: This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.

生物信息
免疫原品种	His-tagged green fluorescent protein of Aequorea victoria.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Chicken
特异性	Recognizes green fluorescent protein (GFP), Mr 30 kDa, and GFP-fusion proteins.
同种型	IgY
品种反应性	Vertebrates
Species Reactivity Note	Vertebrates
抗体类型	Polyclonal Antibody
基因符号	GFP
纯化方法	Ammonium Sulfate Precipitation and PEG
UniProt编号	P42212
UniProt汇总	FUNCTION: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. BIOPHYSICOCHEMICAL PROPERTIES: Absorption: Abs(max)=395 nm; Note=Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm; SUBUNIT: Monomer. TISSUE SPECIFICITY: Photocytes. PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. BIOTECHNOLOGY: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. BIOTECHNOLOGY: Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. SIMILARITY: Belongs to the GFP family.

产品使用声明
质量保证	Routinely evaluated by Western Blot on rat brain lysate containing purified GFP (Catalog #14-392) or a GFP-fusion protein from a transfected E. coli cell lysate. Western Blot Analysis: 0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Routinely evaluated by Western Blot on rat brain lysate containing purified GFP (Catalog #14-392) or a GFP-fusion protein from a transfected E. coli cell lysate.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.