merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB3118,Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11

  • merck millipore,默克密理博,MAB3118,Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11

    产品名称：Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11
    产品型号：MAB3118
    Detect DYKDDDDK (FLAGepitope tag) using this Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11 validated for use in ELISA, IC, IF, IP & WB.

    merck millipore,默克密理博,MAB3118,Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11

  • 产品介绍
  • merck millipore,默克密理博,MAB3118,Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    E. coli, VrtWB, ELISA, ICCMAscitesMonoclonal Antibody
    描述
    产品目录编号 MAB3118
    Replaces AB3250
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11
    背景信息 Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

    FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
    产品信息
    格式 Ascites
    控制
    • Cells transfected with FLAG-tagged fusion vector
    演示 Ascites mouse monoclonal IgG1 liquid containing no preservative.
    应用
    应用 Detect DYKDDDDK (FLAGepitope tag) using this Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11 validated for use in ELISA, IC, IF, IP & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunocytochemistry
    应用说明 Immunofluorescence:
    A 1:1,000-1:5,000 dilution of a previous lot was used in IF.

    Immunoprecipitation:
    A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.

    ELISA:
    A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
    Note: Does not require Ca++ for binding.

    Immunoblotting:
    on recombinant proteins

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Synthetic peptide (Cys)-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys coupled to ovalbumin
    表位 Epitope tag
    克隆 2EL-1B11
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 DYKDDDDK sequence containing proteins. Reacts with both N-terminal and C-terminal fusion proteins.
    同种型 IgG1
    品种反应性 E. coliVertebrates
    Species Reactivity Note Expected to react with rat based on sequence homology.
    抗体类型 Monoclonal Antibody
    纯化方法 Unpurified
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on Flag-mTor protein blot.

    Western Blot Analysis:
    1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
    Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MAB3118,Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11

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