merck millipore,默克密理博,70-500,EZ-TFA (Universal Transcription Factor Assay, colorimetric)

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Merck Millipore 默克密理愽

品种反应性	主要应用	检测方法
M, Rb	ACT, ELISA	Chromogenic

描述
产品目录编号	70-500
品牌系列	Upstate
商名	Upstate EZ-TFA
描述	EZ-TFA (Universal Transcription Factor Assay, colorimetric)
背景信息	The UPSTATE Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts. This assay combines the principle of the electrophoretic mobility shift assay (EMSA) with the 96-well based enzyme-linked immunosorbent assay (ELISA), enabling manual or high-throughput sample analysis with greater sensitivity than conventional EMSA assays. After certain parameters are optimized, the entire assay takes less than 4 hours to complete with minimal hands-on time. The versatile set up allows for a flexible assay design and because the binding reaction occurs in solution, various parameters can be optimized such as probe titration, competitive oligonucleotide concentration, or treatment conditions. This kit contains the reagents and instructions necessary to develop an assay to monitor the DNA binding activity of any transcription factor of interest, or as a kit component for Upstate developed target specific Transcription Factor Assays. The Universal kit also allows for the design of native promoter capture probe sequences to interrogate specific transcription factor binding.

产品信息
部件	Gt X Ms, HRP Secondary Antibody, Catalog # 2003482 Transcription Factor Assay Plate, Catalog # 90268 Blocking Reagent, Catalog # 90273 10X Annealing Buffer, Catalog # 2007044 Substrate (TMB/E), Catalog # 90348 Stop Solution, Catalog # 60193 TFA Buffer, 5X, Catalog # 90272 Gt X Rb, HRP Secondary Antibody, Catalog # 90276
检测方法	Chromogenic

应用
应用	The Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts.
主要应用	Activity Assay ELISA

生物信息
宿主	Goat
品种反应性	MouseRabbit

产品使用声明
质量保证	Routinely evaluated by performing the EZ-TFA with a target specific module.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Upon receipt, store components at the temperatures indicated on the labels. Various components to be stored at 4°C, -20°C, or -80°C.

包装信息
数量	96 assays

merck millipore,默克密理博,70-500,EZ-TFA (Universal Transcription Factor Assay, colorimetric)

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