merck millipore,默克密理博,GF240,Sema3A Recombinant Protein

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,GF240,Sema3A Recombinant Protein
产品名称：Sema3A Recombinant Protein
产品型号：GF240

merck millipore,默克密理博,GF240,Sema3A Recombinant Protein

产品介绍

merck millipore,默克密理博,GF240,Sema3A Recombinant Protein

描述
产品目录编号	GF240
描述	Sema3A Recombinant Protein
背景信息	Semaphorins are a large family of well conserved proteins that participate in a variety of cell signaling functions. However, they are best known for their role in development and regeneration within the nervous system. These affects have been shown to be primarily mediated by the class 3 semaporins (Sema3). In particular, the Sema3A family member acts as both a chemorepellant for axonal growth and a chemoattractant for dendritic processes1,2. Here we provide recombinant Sema3A protein to facilitate researchers studying neurite outgrowth. The protein produced is an Fc-chimera containing the highly conserved semphorin domain, the Ig-like domain, and the highly positively charged C-terminal region (without the 20 amino acids at the C-terminus)3.

产品信息
演示	Protein A affinity purified recombinant Sema3A protein in PBS buffer.

生物信息
浓缩	0.25 mg/mL
纯度	The purity of the Sema3A protein was also determined by SDS-PAGE. Purified protein (2µg) was run using non-denaturing conditions and then coomassie blue stained. The protein detected was approximately 125kD in size.
品种反应性	RatChickenHumanMouse
基因符号	Hsema-I Hsema-III SEMA1 SEMAD SEMAIII SEMAL SemD coll-1 semaphorin-like
纯化方法	40µl of 0.25mg/ml recombinant protein.
UniProt编号	Q14563
UniProt汇总	FUNCTION: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1 By similarity. SUBCELLULAR LOCATION: SecretedBy similarity. DOMAIN: Strong binding to neuropilin is mediated by the carboxy third of the protein. SEQUENCE SIMILARITIES: Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 PSI domain. Contains 1 Sema domain.

产品使用声明
质量保证	Growth cone collapse testing was carried out to verify recombinant Sema3A protein induces collapse. Briefly, dorsal root ganglion cells were plated and allowed to grow overnight in differentiation media. The next day the media was replaced with differentiation media or differentiation media with 1µg/ml of Sema3A protein. The cells were then incubated for 30 minutes, fixed, and stained with FITC conjugated antibodies specific for phalloidin (Part No. 90228). Representative images of each assay condition are shown below:
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Growth cone collapse testing was carried out to verify recombinant Sema3A protein induces collapse. Briefly, dorsal root ganglion cells were plated and allowed to grow overnight in differentiation media. The next day the media was replaced with differentiation media or differentiation media with 1µg/ml of Sema3A protein. The cells were then incubated for 30 minutes, fixed, and stained with FITC conjugated antibodies specific for phalloidin (Part No. 90228). Representative images of each assay condition are shown below:

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.