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品种反应性 主要应用 宿主 格式 抗体类型 H IHC, WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB2616 描述 Anti-Kindlin-1 Antibody, clone 4A5.14 Alternate Names - kindlin 1
- Kindlin syndrome protein
- UNC-112 related protein 1
- Unc-112-related protein 1
- chromosome 20 open reading frame 42
背景信息 The Kindlin family is a group of focal adhesion proteins that have recently become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. Specifically associated with Kindler syndrome, is the isoform Kindlin-1 which plays a key role in adhering the actin cytoskeleton to the extracellular matrix of basal keratinocytes. Kindlin-1 may also be involved in tumor growth by modulating TGF-beta 1 signaling (Shi, 2007). It is most widely expressed in the brain, kidney, colon, placenta, skeletal muscle, adrenal gland, and prostate. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-Kindlin-1 Antibody, clone 4A5.14 is an antibody against Kindlin-1 for use in WB, IC, IH. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Papachristou, D.J., et al. (2007). Histopathology. 51(4):499–508.)
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Papachristou, D.J., et al. (2007). Histopathology. 51(4):499–508.)生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human Kindlin-1. 表位 Unknown 克隆 4A5.14 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 The antibody recognizes Kindlin-1. 同种型 IgG2aκ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_060141
Entrez基因汇总 This gene encodes a member of the fermitin family, and contains a FERM domain and a pleckstrin homology domain. The encoded protein is involved in integrin signaling and linkage of the actin cytoskeleton to the extracellular matrix. Mutations in this gene have been linked to Kindler syndrome. [provided by RefSeq]. 基因符号 - kindlin-1
- FERMT1
- C20orf42
- DTGCU2
- KIND1
- Kindlerin
- Kindlin-1
- UNC112A
- URP1
- kinderlin
纯化方法 Protein G purfied UniProt编号 - Q9BQL6
UniProt汇总 FUNCTION: Involved in cell adhesion, possibly via its interaction with integrins. May mediate TGF-beta 1 signaling in tumor progression.
SUBUNIT STRUCTURE: Interacts with the cytoplasmic domain of integrins ITGB1 and ITGB3.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cell junction › focal adhesion. Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions. Colocalizes with filamentous actin. Upon TGFB1 treatment, it localizes to membrane ruffles.
TISSUE SPECIFICITY: Expressed in brain, skeletal muscle, kidney, colon, adrenal gland, prostate, and placenta. Weakly or not expressed in heart, thymus, spleen, liver, small intestine, bone marrow, lung and peripheral blood leukocytes. Overexpressed in some colon and lung tumors. In skin, it is localized within the epidermis and particularly in basal keratocytes.
INDUCTION: By TGF-beta 1.
DOMAIN: The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
INVOLVEMENT IN DISEASE: Defects in FERMT1 are the cause of Kindler syndrome [MIM:173650]. Kindler syndrome is an autosomal recessive disorder characterized by neonatal blistering, sun sensitivity, atrophy, abnormal pigmentation and fragility of the skin.
SEQUENCE SIMILARITIES: Belongs to the kindlin family.
Contains 1 FERM domain.
Contains 1 PH domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Kindlin-1 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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