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merck millipore,默克密理博,MAB1568,Anti-Calretinin Antibody, clone 6B8.2
产品名称：Anti-Calretinin Antibody, clone 6B8.2
产品型号：MAB1568
Anti-Calretinin Antibody, clone 6B8.2 detects level of Calretinin & has been published & validated for use in IH & WB.
merck millipore,默克密理博,MAB1568,Anti-Calretinin Antibody, clone 6B8.2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, M WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB1568 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Calretinin Antibody, clone 6B8.2 Alternate Names - calbindin 2, (29kD, calretinin)
- calbindin 2
- (29kD
背景信息 Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc 产品信息 格式 Purified 控制 - Rat brain cortex, Mesothelioma tissue, mouse brain lysate.
演示 Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide. 应用 应用 Anti-Calretinin Antibody, clone 6B8.2 detects level of Calretinin & has been published & validated for use in IH & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry:
On rat brain tissue:
IFA: 1:1,000-1:5,000
PAP: 1:1,000-1:5,000
The antibody has been used successfully on formalin-fixed, paraffin embedded human tissue after pre-treatment of the tissue slides with citrate buffer and heat treatment. Suggested starting dilution 1:100-1:400. Proteolytic digestion with pepsin is detrimental to the immunostaining.
Western blot:
1:1,000-1:3,000
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Recombinant rat calretinin (Heinze, L., 2007). 克隆 6B8.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Calretinin is a neuron specific calcium binding protein (31 kDa) found mostly in the CNS and retina. It is present in subsets of neurons throughout the brain and spinal cord, including sensory ganglia. It shares about 55% amino acid homology with calbindin, another calcium binding protein found in brain and periphery. Studies suggest that calretinin, like calbindin, may be neuroprotective. 同种型 IgG1 品种反应性 RatMouse Species Reactivity Note Mouse and rat. Other species have not been tested. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001740.3
Entrez基因汇总 Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin 2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin 1. Calbindin 2 contains five presumably active and one presumably inactive calcium-binding domains. Comparison with the partial sequences available for chick and guinea pig calbindin 2 reveals that the protein is highly conserved in evolution. The calbindin 2 message was detected in the brain, while absent from heart muscle, kidney, liver, lung, spleen, stomach and thyroid gland. There are two additional forms of alternatively spliced calbindin 2 mRNAs encoding C-terminally truncated proteins. Exon 7 can splice to exon 9, resulting in a frame shift and a translational stop at the second codon of exon 9, and encoding calretinin-20k. Exon 7 can also splice to exon 10, resulting in a frame shift and a translational stop at codon 15 of exon 10, and encoding calretinin-22k. The truncated proteins are able to bind calcium. 基因符号 - CAL2
- CALB2
- Calretinin
- CAB29
- CR
纯化方法 Protein A purfied UniProt编号 - P22676
UniProt汇总 FUNCTION: SwissProt: P22676 # Calretinin is a calcium-binding protein which is abundant in auditory neurons.
SIZE: 271 amino acids; 31540 Da
TISSUE SPECIFICITY: Brain.
SIMILARITY: SwissProt: P22676 ## Belongs to the calbindin family. & Contains 6 EF-hand domains.产品使用声明 质量保证 Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Calretinin on 10 μg of mouse brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,ABC283,Anti-MGEA5 Antibody
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