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merck millipore,默克密理博,MAB2514,Anti-Integrin α5β1 Antibody, clone BMB5
产品名称：Anti-Integrin α5β1 Antibody, clone BMB5
产品型号：MAB2514
This Anti-Integrin α5β1 Antibody, clone BMB5 is validated for use in FC, IP, WB, IH, FUNC for the detection of Integrin α5β1.
merck millipore,默克密理博,MAB2514,Anti-Integrin α5β1 Antibody, clone BMB5
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M IHC, FC, WB R Ascites Monoclonal Antibody 描述 产品目录编号 MAB2514 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin α5β1 Antibody, clone BMB5 Alternate Names - VLA-5
产品信息 格式 Ascites 演示 Liquid, containing no preservatives. 应用 应用 This Anti-Integrin α5β1 Antibody, clone BMB5 is validated for use in FC, IP, WB, IH, FUNC for the detection of Integrin α5β1. 主要应用 - Immunohistochemistry
- Flow Cytometry
- Western Blotting
应用说明 Inhibition of cell adhesion to fibronectin: 10 μg/mL
Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMB5 alone may not be complete.
Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μg
Flow cytometry: 10μg/mL
Western blot (non-denaturing gel)
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. 克隆 BMB5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 特异性 Antibody MAB2514 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions. 同种型 IgG3 品种反应性 Mouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002205.2
Entrez基因汇总 The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. 基因符号 - FNRA
- ITGA5
- VLA-5
- CD49e
- VLA5A
UniProt编号 - P08648
UniProt汇总 FUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1049 amino acids; 114536 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µL
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