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品种反应性 主要应用 宿主 格式 抗体类型 H ICC, ELISA, FC, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB8345 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MMP-14 Antibody Alternate Names - MT1-MMP
产品信息 格式 Serum 控制 - Immunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.
Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma
演示 Liquid in PBS, pH 7.6, containing 0.01% sodium azide as a preservative. 应用 应用 Anti-MMP-14 Antibody detects level of MMP-14 & has been published & validated for use in ELISA, FC, IP, WB & IC. 主要应用 - Immunocytochemistry
- ELISA
- Flow Cytometry
- Western Blotting
应用说明 Immunocytochemistry: 5-10 μg / ml
Western blot: 5 μg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.
Immunopreciptation: 1-2 μg / mg total protein in 500 μl reaction volume.
FACS Analysis: 5 μg/ mL (1 million cells per mL)
EIA: 1:10,000
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Catalytic domain of MT1-MMP 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody recognizes native and recombinant proteins. 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004995.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. 基因符号 - EC 3.4.24.80
- MMP14
- MTMMP1
- MMP-14
- MT1MMP
- MT1-MMP
- MMP-X1
UniProt编号 - P50281
UniProt汇总 FUNCTION: SwissProt: P50281 # Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity).
SIZE: 582 amino acids; 65884 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
DOMAIN: SwissProt: P50281 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store antibody at -20°C for 12 months. Avoid repeat freeze thaw cycles. 包装信息 数量 100 µL
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