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品种反应性 主要应用 宿主 格式 抗体类型 R, M WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB2226 描述 Anti-Piccolo Antibody, clone 4G3.3 Alternate Names - Aczonin
- presynaptic cytomatrix protein
背景信息 Piccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE]. 产品信息 格式 Purified 控制 - Rat brain lysate, mouse cerebellum
演示 Purified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide. 应用 应用 This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo. 主要应用 - Western Blotting
应用说明 Immunohistochemistry (Paraffin):
Representative images from a previous lot.
Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).
生物信息 免疫原品种 GST-tagged recombinant protein 克隆 4G3.3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes piccolo. 同种型 IgMκ 品种反应性 RatMouse Species Reactivity Note Mouse and Rat.
Predicted to react with human based on 82% sequence homology and rat based on 92% sequence homology.
抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001104266
基因符号 - KIAA0559
- ACZ
- Aczonin
- DKFZp779G1236
纯化方法 Ammonium sulfate precipitation UniProt编号 - Q9Y6V0
UniProt汇总 FUNCTION: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking (By similarity).
SUBUNIT: Interacts with Rabac1/Pra1, RIMS2 and profilin (By similarity).
SUBCELLULAR LOCATION: Cell junction, synapse (By similarity). Note=Concentrated at the presynaptic side of synaptic junctions (By similarity).
ALTERNATIVE PRODUCTS: 3 named isoforms
DOMAIN: C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
SIMILARITY: Contains 2 C2 domains.
SIMILARITY: Contains 1 PDZ (DHR) domain.
SEQUENCE CAUTION:
Sequence=AAD21789.2; Type=Erroneous gene model prediction;产品使用声明 质量保证 Routinely evaluated by western blot on rat brain lysate. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,428021,Latrunculin A, Latrunculia magnifica - CAS 76343-93-6 - Calbiochem
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