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merck millipore,默克密理博,MAB19562,Anti-Laminin-5 (γ2 chain) Antibody, clone D4B5
产品名称：Anti-Laminin-5 (γ2 chain) Antibody, clone D4B5
产品型号：MAB19562
Anti-Laminin-5 (γ2 chain) Antibody, clone D4B5 is an antibody against Laminin-5 (γ2 chain) for use in ELISA, IH(P) & WB.
merck millipore,默克密理博,MAB19562,Anti-Laminin-5 (γ2 chain) Antibody, clone D4B5
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, ELISA M Purified Monoclonal Antibody 描述 产品目录编号 MAB19562 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Laminin-5 (γ2 chain) Antibody, clone D4B5 Alternate Names - Laminin 5
背景信息 Laminins are basement membrane, extracellular matrix glycoproteins that are composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes. Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease. LN5 may stabilize the epidermal/dermal junction of skin through binding with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. Compared to fibronectin, collagen, or vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent. Recently, Laminin-5 is attracting attention as a substrate for carcinoma cells that may stimulate migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, et al., 2002, Lohi, et al., 2001). 产品信息 格式 Purified 控制 - Human pancreatic tumor tissue, human breast carcinoma tissue or A-431 whole cell lysate
演示 Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. 应用 应用 Anti-Laminin-5 (γ2 chain) Antibody, clone D4B5 is an antibody against Laminin-5 (γ2 chain) for use in ELISA, IH(P) & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 5ug/ml is recommended; dilute antibody in PBS or TBS only, not blocking solutions; blocking with purified compounds is suggested.
Immunohistochemistry of paraffin sections: 5-10ug/ml is suggested. Do not over fix. A light 4% or 2% PFA are best. 4 mm thick paraffin sections are dewaxed, rehydrated and immersed in 0.3% hydrogen peroxide-containing methanol for inactivation of intrinsic peroxidase, followed by treatment with Protease XXIV (Sigma) for 15 min at room temperature (0.01-0.05%). Do not use trypsin. Antibody incubation at 37°C for 1 hour, followed by secondary antibody detection and visualization with DAB (Mizushima et al. 1998). Do not place the monoclonal antibody in serum blocks because serum conatins laminin 5 which will bind and interfere with the antibody. Use BSA or other such media instead.
Strongly positive staining is seen in Basement Membrane of the following tissues: Skin, Esophagus, Lung, and Kidney. Positive staining is also seen in Stomach, Small intestine, Spleen, Thymus, Prostate, and Ovary. Thyroid, Testis, Skeletal and Cardiac muscle were negative for LN5 staining.
Inhibition of alpha2beta1 integrin binding to laminin 5 (40ug/ml) (Decline & Rousselle 2000).
ELISA Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Amino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein 克隆 D4B5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Monoclonal antibody MAB19562 was developed against amino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein. The antibody recognizes the 150 (precursor form) and 105 kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5. 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_018891.2
- NM_005562.2
基因符号 - CSF
- LAMC2
- EBR2
- LAMB2T
- MGC141938
- MGC138491
- LAMNB2
- epiligrin
- BM600-100kDa
- BM600
- kalinin-105kDa
- nicein-100kDa
- EBR2A
- B2T
纯化方法 Protein A purfied UniProt编号 - Q13753
UniProt汇总 FUNCTION: SwissProt: Q13753 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell- scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.
SIZE: 1193 amino acids; 130976 Da
SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Gamma-2 is a subunit of laminin-5 (epiligrin/kalinin/nicein).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
TISSUE SPECIFICITY: The large variant is expressed only in specific epithelial cells of embryonic and neonatal tissues. In 17-week old embryo the small variant is found in cerebral cortex, lung, and distal tubes of kidney, but not in epithelia except for distal tubuli.DOMAIN:SwissProt: Q13753 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domain IV is globular.
DISEASE: SwissProt: Q13753 # Defects in LAMC2 are a cause of junctional epidermolysis bullosa gravis (JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB is a blistering disorder in skin that is characterized by a separation of basal cells from the basement membrane due to a decreased number of hemidesmosomes. Laminin-5 is missing from the basement membrane of patients with the gravis form of epidermolysis bullosa.
SIMILARITY: Contains 8 laminin EGF-like domains. & Contains 1 laminin IV type A domain.
MISCELLANEOUS: Binds heparin (By similarity).产品使用声明 质量保证 Evaluated by Western Blot on recombinant Laminin-5.
Western Blot Analysis: A 1:500-1:2000 dilution of this antibody detected Laminin-5, gamma2 chain in 10 µg of human recombinant Laminin-5.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µg
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