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merck millipore,默克密理博,04-1467,Anti-PABP 1 Antibody, clone 10E10
产品名称：Anti-PABP 1 Antibody, clone 10E10
产品型号：04-1467
Anti-PABP 1, clone 10E10 is a Mouse Monoclonal Antibody for detection of PABP 1 also known as PABP 1, Poly(A)-binding protein 1, poly(A) binding protein cytoplasmic 1 & has been validated in WB, ICC & More>>
merck millipore,默克密理博,04-1467,Anti-PABP 1 Antibody, clone 10E10
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 04-1467 Replaces 05-847 描述 Anti-PABP 1 Antibody, clone 10E10 Alternate Names - poly(A)-binding protein, cytoplasmic 2
- PABP 1
- Poly(A)-binding protein 1
- poly(A) binding protein, cytoplasmic 1
- poly(A) binding protein, cytoplasmic 2
背景信息 Poly(A)-binding protein (PABP 1) is an abundant protein that interacts with the 3' poly(A) tail of most eukaryotic mRNAs. In vivo this protein is involved in many aspects of mRNA metabolism, including mRNA stability, translationally coupled mRNA turnover, protein biosynthesis, and ribosome biogenesis. PABP1 shuttles between the cytoplasm and the nucleus and may bind to cytoplasmic RNA sequences other than poly(A). PABP1 accumulates in the nucleus if transcription is inhibited, suggesting that nuclear export is coupled to active transcription. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-PABP 1, clone 10E10 is a Mouse Monoclonal Antibody for detection of PABP 1 also known as PABP 1, Poly(A)-binding protein 1, poly(A) binding protein cytoplasmic 1 & has been validated in WB, ICC & IP. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunoprecipitation:
Cited in Thoma, C., et al. (2008) RNA. 14:1579-1589, and Buchet-Poyau, K., et al.(2007) Nuc. Acids Res. 35:1289-1300 using a representative lot.
Immunocytochemistry:
Cited in Afonina, E, et al. (1998) J. Biol. Chem., 273: 13015-21 (1998) using a representative lot.生物信息 免疫原品种 Recombinant protein corresponding to human PABP 1. 表位 Unknown 克隆 10E10 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes PABP 1. 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002559.2
Entrez基因汇总 The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.[supplied by OMIM] 基因符号 - PABPL1
- PABPC1
- PAB1
- PABP
- PABP1
- PABPC2
纯化方法 Protein G purfied UniProt编号 - P11940
UniProt汇总 FUNCTION: Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre- mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
SIZE: 636 amino acids; 70671 Da
SUBUNIT: Component of a multi subunit autoregulatory ribonucleoprotein complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1. Interacts with IGF2BP1. Part of a complex associated with the FOS mCRD domain and consisting of HNRPD, SYNCRIP, PAIP1 and CSDE1/UNR. Interacts with the PABPC1- interacting motif-1 (PAM1) and -2 (PAM2) of PAIP1 and PAIP2. Interacts with PAIP1 with a 1:1 stoichiometry and with PAIP2 with a 1:2 stoichiometry. The interaction with CSDE1 is direct and RNA- independent. Found in a mRNP complex with YBX2. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus.
TISSUE SPECIFICITY: Ubiquitous.
DOMAIN: The RNA-binding domains RRM1 and RRM2 and the C-terminus (last 138 amino acids) regions interact with the PABPC1- interacting motif-1 (PAM1) and -2 (PAM2) of PAIP1, respectively. & The RNA-binding domains RRM2 and RRM3 and the C-terminus (last 138 amino acids) regions interact with the PABPC1- interacting motif-1 (PAM1) and -2 (PAM2) of PAIP2, respectively.
PTM: Methylated by CARM1. & Arg-493 is dimethylated, probably to asymmetric dimethylarginine.
SIMILARITY: Contains 1 PABC domain. & Contains 4 RRM (RNA recognition motif) domains.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected PABP 1 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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