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重要规格表
检测方法 Colorimetric 描述 概述 A quantitative colorimetric assay for the measurement of Bcl-2. Able to detect significant decreases in Bcl-2 protein levels before significant levels of apoptosis are detected. Kit has high sensitivity, a broad dynamic range, and contains all required buffers. One unit of Bcl-2 equals the Bcl-2 activity in 5.6 X 104 HL-60 cells 产品目录编号 QIA23 品牌系列 Calbiochem®
应用数据 
Neutralization of bcl-2 positive samples using a bcl-2 monoclonal antibody (Cat. No. OP60).
The pooled coefficient of variation (according to the formula of Henry, et al., 1974) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.
The pooled coefficient of variation (according to the formula of Henry, et al., 1975) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.
Human sera and plasma samples were spiked with bcl-2 protein to obtain a final concentration of 42 units/ml and analyzed as outlined above. The control represents bcl-2 spiked into sample diluent.
Dilution recovery from a bcl-2 positive cell line using different antigen extraction agents (different detergents).
Dilution recovery using different cell lines.参考 参考 Korsmeyer, S.J., 1995. Trends Genet. 11, 101.
Hockenbery D.M., 1995. BioEssays 17, 631.
Steinman, H.M., 1995. J. Biol. Chem. 270, 3487.
Hawkins, C.J., and Vaux, D., 1994. Immunol. Rev. 142, 127.
Hengartner, M.O., et al. 1994. Nature 369, 318.
Ellis, R.E., et al. 1994. Soc. London Ser. 34, 243.
Zoltan, N., et al. 1994. Cell 79, 189.
Tsujimoto, Y. 1989. Oncogene 4, 1331.产品信息 检测方法 Colorimetric 形式 96 Tests 格式 96-well plate 试剂盒包含 96 Well Coated Plate, Antigen Extraction Reagent, Lyophilized Bcl-2 Standard, Sample Diluent, Anti-FITC Conjugated Detector Antibody, Concentrated Anti-FITC Peroxidase Conjugate, Conjugate Diluent, Substrate, Wash Buffer, Stop Solution, Adhesive Plate Sealers, and a user protocol. 应用 主要应用 Enzyme-Linked Immunosorbent Assay
生物信息 化验时间 3.5 h 样品类型 Cell lysates, tissue culture supernatant, sera, or plasma 品种 human 理化信息 灵敏度 < 1 ng/ml 安全信息 R相 R: 23/24/25-35-36/37/38-40-43
Toxic by inhalation, in contact with skin and if swallowed.
Causes severe burns.
Irritating to eyes, respiratory system and skin.
Limited evidence of a carcinogenic effect.
May cause sensitization by skin contact.S相 S: 26-36/37/39-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).产品使用声明 预定用途 The Calbiochem® bcl-2 ELISA is a non-isotopic immunoassay for the in vitro quantitation of human bcl-2 protein in cell extracts, tissue culture medium, sera and plasma. This assay is not recommended for use with rodent samples. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 -20°C
存储条件 Upon receipt, standards must be stored at -20°C. All other components may be stored at -20°C or 4°C (do not refreeze after thawing). Do not expose reagents to excessive light. Allow kit reagents to warm to room temperature before use. 避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 96 Well Coated Plate, Antigen Extraction Reagent, Lyophilized Bcl-2 Standard, Sample Diluent, Anti-FITC Conjugated Detector Antibody, Concentrated Anti-FITC Peroxidase Conjugate, Conjugate Diluent, Substrate, Wash Buffer, Stop Solution, Adhesive Plate Sealers, and a user protocol.
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