merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB1947,Anti-Laminin-5 Antibody, clone P3H9-2

  • merck millipore,默克密理博,MAB1947,Anti-Laminin-5 Antibody, clone P3H9-2

    产品名称：Anti-Laminin-5 Antibody, clone P3H9-2
    产品型号：MAB1947
    Detect Laminin-5 using this Anti-Laminin-5 Antibody, clone P3H9-2 validated for use in ELISA, IP, WB, IC, IH.

    merck millipore,默克密理博,MAB1947,Anti-Laminin-5 Antibody, clone P3H9-2

  • 产品介绍
  • merck millipore,默克密理博,MAB1947,Anti-Laminin-5 Antibody, clone P3H9-2

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HIHC, ELISA, WB, ICCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB1947
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Laminin-5 Antibody, clone P3H9-2
    Alternate Names
    • Epiligrin
    背景信息 Epiligrin, the major component of human keratinocyte extracellular matrix, serves as the preferred integrin ligand for alpha 3 beta 1 in plasma membranes and focal adhesions, and colocalizes with alpha 6 beta 4 in hemidesmosomes. {Domloge-Hultsch, et al (1992) J Clin Invest 90(4):1628-1633}. Major glycoprotein of epidermal basement membrane, consisting of three disulphide bonded subunits of 170, 145 and 135 kD. Epiligrin is the major ligand for alpha3/beta1 integrin, is particularly prominent in the lamina lucida of the skin and is absent in patients with lethal junctional epidermolysis bullosa. Today, epiligrin is identical to laminin 5, also called BM600, nicein, and kalinin. Laminin 5 (epiligrin; Carter et al, 1991 Cell 65(4):599-610) as the primary ligand in epithelial BMs that mediates both cell anchorage (adhesion without migration) via integrin a6b4 in homeostatic tissue and cell migration via integrin a3b1 in wound repair.

    The laminin-5 isoform (nicein, epiligrin, and kalinin) is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa, and other epithelial glands (Kallunki et al., 1992; Stahl et al., 1997). Laminin-5 is a heterotrimer consisting of alpha3, beta3, and gamma2 subunits that associate via large helical regions to produce a cruciform-shaped molecule (Rousselle et al., 1991 J. Cell Biol. 114: 567-576; Baker et al., 1996 J. Cell Sci. 109: 2509-2520). Laminin-5 is synthesized initially as a 460-kD molecule that undergoes specific processing to a smaller form after being secreted into the extracellular matrix (Marinkovich et al., 1992 J. Biol. Chem. 267: 17900-17906; Vailly et al., 1994 Eur. J. Biochem. 219: 209-218; Matsui et al., 1995 J. Biol. Chem. 270: 23496-23503). The size reduction is a result of processing the 3 and 2 subunits from 190-200 to 160 kD and from 155 to 105 kD, respectively (Marinkovich et al., 1992; Vailly et al., 1994; Matsui et al., 1995). {Goldfinger, LE (1998) J Cell Biol 141(1):255-265}.
    产品信息
    格式 Purified
    演示 Liquid in 0.1M PBS. pH=7.1, no preservatives.
    应用
    应用 Detect Laminin-5 using this Anti-Laminin-5 Antibody, clone P3H9-2 validated for use in ELISA, IP, WB, IC, IH.
    主要应用
    • Immunohistochemistry
    • ELISA
    • Western Blotting
    • Immunocytochemistry
    应用说明 Immunohistochemistry: for use on acetone fixed tissue

    Immunocytochemistry

    Immunoblotting: (non-reducing)

    Immunoprecipitation

    Inihibition of laminin 5 binding

    ELISA

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Human keratinocytes
    克隆 P3H9-2
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Human epiligrin (laminin 5)
    同种型 IgG1
    品种反应性 Human
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_018891.2
    • NM_005562.2
    基因符号
    • LAMC2
    • EBR2
    • LAMB2T
    • MGC141938
    • MGC138491
    • LAMNB2
    • epiligrin
    • BM600-100kDa
    • BM600
    • kalinin-105kDa
    • nicein-100kDa
    • EBR2A
    • B2T
    • CSF
    UniProt编号
    • Q13753
    UniProt汇总 FUNCTION: SwissProt: Q13753 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell- scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.
    SIZE: 1193 amino acids; 130976 Da
    SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Gamma-2 is a subunit of laminin-5 (epiligrin/kalinin/nicein).
    SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
    TISSUE SPECIFICITY: The large variant is expressed only in specific epithelial cells of embryonic and neonatal tissues. In 17-week old embryo the small variant is found in cerebral cortex, lung, and distal tubes of kidney, but not in epithelia except for distal tubuli.DOMAIN:SwissProt: Q13753 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domain IV is globular.
    DISEASE: SwissProt: Q13753 # Defects in LAMC2 are a cause of junctional epidermolysis bullosa gravis (JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB is a blistering disorder in skin that is characterized by a separation of basal cells from the basement membrane due to a decreased number of hemidesmosomes. Laminin-5 is missing from the basement membrane of patients with the gravis form of epidermolysis bullosa.
    SIMILARITY: Contains 8 laminin EGF-like domains. & Contains 1 laminin IV type A domain.
    MISCELLANEOUS: Binds heparin (By similarity).
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Store at +2 and 8°C under sterile conditions for up to 6 months.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB1947,Anti-Laminin-5 Antibody, clone P3H9-2

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