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品种反应性 主要应用 宿主 格式 抗体类型 Rb, B, Gp, H, M, R WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB13446 Replaces MAB13448 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-TIMP-2 Antibody, clone 2TMP05 Alternate Names - Tissue Inhibitor of Metalloproteinase-2
产品信息 格式 Purified 控制 - POSITIVE CONTROL:
Pancreatic or colon carcinoma
演示 Purified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. 应用 应用 Detect TIMP-2 using this Anti-TIMP-2 Antibody, clone 2TMP05 validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistology (formalin/paraffin only): 2-4μg/ml 30 min at RT.
Western blotting: 1μg/ml for 2 hrs at RT.
(Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min).
The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator.生物信息 免疫原品种 A synthetic peptide from the N-terminal region of human TIMP-2 protein. 克隆 2TMP05 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 MAB13446 recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinase-2 (TIMP-2). TIMP-2 is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. CHEMICON MAB13446 may be used to evaluate the invasive nature of some tumor types and determine the tissue distribution of TIMPs in other tumors such as adenomas and also its normal tissue.
CELLULAR LOCALIZATION:
Cytoplasmic品种反应性 RabbitBovineGuinea PigHumanMouseRat 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_003255.4
Entrez基因汇总 This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. 基因符号 - CSC-21K
- TIMP2
- TIMP-2
UniProt编号 - P16035
UniProt汇总 FUNCTION: SwissProt: P16035 # Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
SIZE: 220 amino acids; 24399 Da
SUBCELLULAR LOCATION: Secreted.
PTM: The activity of TIMP2 is dependent on the presence of disulfide bonds.
SIMILARITY: SwissProt: P16035 ## Belongs to the protease inhibitor I35 (TIMP) family. & Contains 1 NTR domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Antibody with sodium azide is stable for 12 months after date of receipt when stored at 2-8°C. 包装信息 数量 100 µg
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