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merck millipore,默克密理博,HTS110M,ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT4B Serotonin Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT4B Serotonin Receptor
产品型号：HTS110M
Human 5-HT4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS110M,ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT4B Serotonin Receptor
- 产品介绍
- merck millipore,默克密理博,HTS110M,ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT4B Serotonin Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 5-HT4 描述 产品目录编号 HTS110M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT4B Serotonin Receptor 概述 Human HTR4, transcript b, encoding 5-HT4B 背景信息 5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT4 comprises at least 8 isoforms varying at the C-terminus, which are generated by alternative splicing. The expression and distribution of these splice variants differs among organs and tissues with many of them present in several tissues such as atrium, brain, and GI tract (Bockaert et al. 2004). To date, all isoforms have been shown to activate adenylyl cyclase in vitro, and no difference in signal transduction between C-terminal 5-HT4 receptor variants has been demonstrated. 5-HT4 receptors are of potential interest for the treatment of patients with GI motility disorders and Alzheimer's disease (De Maeyer et al. 2008; Lezoualc’h 2007). Millipore's 5-HT4B membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of 5-HT4B. The membrane preparations exhibit a Kd of 0.46 nM for [3H]-GR113808. With 1 nM [3H]-GR113808, 5 μg/well 5-HT4B Membrane Prep yields greater than 10 fold signal-to-background ratio. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human 5-HT4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_000870
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 5-HT4 靶子系列 Serotonin 孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in assay buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with assay buffer. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with assay buffer. The plate is dried and counted.
Assay buffer: 25 mM Tris, pH 7.4, filtered and stored at 4°C
Radioligand: [3H]-GR113808 (Amersham TRK944)
One package contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 10-fold signal:background with 3H-labeled GR113808 at 1 nM.产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of 5-HT4B membrane prep.
10 µg/well 5 µg/well Signal:Background 26.4 21.7 Specific Binding (cpm) 5286 4879
SPECIFICATIONS: 1 unit = 5 μg membrane preparation
Bmax: 12.5 pmol/mg
Kd: 2.7 nM for [3H]-GR113808
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw. 包装信息 数量 200 units
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