merck millipore,默克密理博,04-1103,Anti-E-cadherin Antibody, clone EP700Y, rabbit monoclonal

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merck millipore,默克密理博,04-1103,Anti-E-cadherin Antibody, clone EP700Y, rabbit monoclonal
产品名称：Anti-E-cadherin Antibody, clone EP700Y, rabbit monoclonal
产品型号：04-1103
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect E-cadherin using this Anti-E-cadherin Antibod More>>

merck millipore,默克密理博,04-1103,Anti-E-cadherin Antibody, clone EP700Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1103,Anti-E-cadherin Antibody, clone EP700Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1103
描述	Anti-E-cadherin Antibody, clone EP700Y, rabbit monoclonal
Alternate Names	cell-CAM 120/80 CAM 120/80 CD324 antigen cadherin 1, E-cadherin (epithelial) cadherin 1, type 1 cadherin 1, type 1, E-cadherin (epithelial) calcium-dependent adhesion protein, epithelial
背景信息	Cadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.

产品信息
格式	Unpurified
控制	MCF-7 cell lysates.
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect E-cadherin using this Anti-E-cadherin Antibody, clone EP700Y, Rabbit validated for use in IH(P), WB, IC & IP.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected E-cadherin in human breast carcinoma and hepatocellular carcinoma tissue. Immunocytochemistry: 1:500 dilution from a previous lot was used on IC. Immunoprecipitation: 1:50 dilution from a previous lot was used on IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues in the 5th cadherin domain of human E-cadherin.
克隆	EP700Y
宿主	Rabbit
特异性	This antibody recognizes E-cadherin.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	P12830
基因符号	uvomorulin Arc-1 CD324 CDHE Cadherin-1 E-cadherin ECAD LCAM UVO Uvomorulin
纯化方法	Unpurified
UniProt编号	P12830
UniProt汇总	FUNCTION: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. SUBUNIT STRUCTURE: Homodimer; disulfide-linked. Interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLGAP5. SUBCELLULAR LOCATION: Cell junction. Cell membrane; Single-pass type I membrane protein. Note: Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. TISSUE SPECIFICTIY: Non-neural epithelial tissues. PTM: During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions. INVOLVEMENT IN DISEASE: Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Defects in CDH1 are a cause of gastric cancer [MIM:137215]; also known as hereditary familial diffuse gastric cancer (HDGC). Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089]. Defects in CDH1 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome. SEQUENCE SIMILARITIES: Contains 5 cadherin domains. SEQUENCE CAUTION: The sequence AAA61259.1 differs from that shown. Reason: Frameshift at positions 16, 22, 25, 28, 31, 34, 52, 67, 73, 76, 94, 102, 633 and 636.

产品使用声明
质量保证	Evaluated by Western Blot on MCF-7 cell lysates. Western Blot Analysis: 1:50,000 - 200,000 dilution of this antibody was used to detect E-cadherin in MCF-7 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.